1887

Abstract

SUMMARY

The presence of lipids has been demonstrated in mycobacteriophage 13. The total lipid was composed of 69% phospholipids and 31% neutral lipids. More than two-thirds of phospholipids present in the phage were synthesized in the host prior to infection. The fatty acid composition of the phage differed markedly from that of its host, both in chain length and the degree of saturation. The phage lipid was mostly composed of saturated fatty acids of which more than 50% were short chain fatty acids. Changes in growth temperatures reflected variations in fatty acid composition, characteristic of the phage, and which were distinctly different from those of the host. Electron microscopic observations revealed that the phage has a membranous bilayer structure. The presence of lipids may facilitate the phage-host interaction especially in lipid-rich organisms like mycobacteria.

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1982-03-01
2024-12-13
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References

  1. Amako K., Yasunaka K. 1977; Ether induced morphological alteration of pf-1 filamentous phage. Nature, London 267:862–863
    [Google Scholar]
  2. Bartlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemical Physiology 37:911–917
    [Google Scholar]
  4. Bowman B. U., Newman H. A. L., Moritz J. M., Koehler R. M. 1973; Properties of mycobacteriophage DS6A. American Review of Respiratory Diseases 107:42–49
    [Google Scholar]
  5. Bradley D. E., Rutherford E. L. 1975; Basic characterization of a lipid containing bacteriophage specific for plasmids P, N and W compatibility groups. Canadian Journal of Microbiology 21:152–163
    [Google Scholar]
  6. Bragdon J. H. 1951; Colorimetric determination of blood lipids. Journal of Biological Chemistry 190:513–515
    [Google Scholar]
  7. Braunstein S. N., Franklin R. M. 1971; Structure and synthesis of a lipid containing bacteriophage. V. Phospholipids of the host BAL-31 and of the bacteriophage PM2. Virology 43:685–695
    [Google Scholar]
  8. Burton K. 1968; Determination of DNA concentration with diphenylamine. Methods in Enzymology 12B:163–166
    [Google Scholar]
  9. Day M. F., Mercer E. H. 1964; Properties of an iridescent virus from the beetle Sericesthis pruinosa. Australian Journal of Biological Sciences 17:892–902
    [Google Scholar]
  10. Duncombe W. G. 1963; The colorimetric microdetermination of long chain fatty acids. Biochemical Journal 88:7–10
    [Google Scholar]
  11. Harrison S. C., Caspar D. L. D., Camerini-Otero R. D., Franklin R. M. 1971; Lipid and protein arrangements in bacteriophage PM2. Nature New Biology 229:197–201
    [Google Scholar]
  12. Jones W. D., David H. L., Beam R. E. 1970; The occurrence of lipids in mycobacteriophage D29 propagated in Mycobacterium smegmatis ATCC 607. American Review of Respiratory Diseases 102:814–817
    [Google Scholar]
  13. Karnik S. S., Gopinathan K. P. 1980; Possible involvement of calcium stimulated ATP-hydrolysing activity associated with mycobacteriophage 13 in the DNA injection process. Journal of Virology 33:969–975
    [Google Scholar]
  14. Kelly D. C., Vance D. E. 1973; The lipid content of two iridescent viruses. Journal of General Virology 21:417–423
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Marinetti G. V. 1962; Chromatographic separation, identification and analysis of phosphatides. Journal of Lipid Research 3:1–17
    [Google Scholar]
  17. Nagaraja V., Gopinathan K. P. 1980; Requirement for calcium ions in mycobacteriophage 13 DNA injection and propagation. Archives of Microbiology 124:249–254
    [Google Scholar]
  18. Sands J. A. 1973; The phospholipid composition of bacteriophage ϕ 6. Biochemical and Biophysical Research Communications 55:111–116
    [Google Scholar]
  19. Sands J. A. 1976; Origin of the phospholipids of a lipid containing virus that replicates in E. coli: bacterio-phage PR4. Journal of Virology 19:296–301
    [Google Scholar]
  20. Schafer R., Huber H., Franklin R. M. 1977; Chemical and physical properties of mycobacteriophage D29. European Journal of Biochemistry 73:239–246
    [Google Scholar]
  21. Sellers M. I., Tokunaga T. 1970; Inactivation of mycobacteriophage by lipid solvents. In Host-Virus Relationships in Mycobacterium Nocardia and Actinomycetes pp 134–143 Springfield, Illinois: Charles C. Thomas;
    [Google Scholar]
  22. Soloff B. L., Rado T. A., Henry B. E. II, Bates J. H. 1978; Biochemical and morphological characterization of mycobacteriophage RI. Journal of Virology 25:253–262
    [Google Scholar]
  23. Vidaver A. K., Koski R. K., van Etten J. L. 1973; Bacteriophage ϕ 6: a lipid containing virus of Pseudomonas phaseolicola. Journal of Virology 11:799–805
    [Google Scholar]
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