1887

Abstract

SUMMARY

Human cytomegalovirus (HCMV) DNA was digested with restriction endonucleases and the fragments characterized with respect to molecular weight and relative mole proportions. The terminal fragments were identified by digesting HCMV DNA with exonucleases before restriction endonuclease treatment and subsequent gel analysis. The dIII fragments of HCMV DNA were cloned in and recombinant plasmids were characterized by digestion with restriction endonucleases and by molecular hybridization with dIII, II and I fragments of the virus genome. Data from these experiments were used to construct physical maps of HCMV DNA for the dIII, II and I restriction endonucleases. The terminal regions of the genome and the region containing fragment dIII M were shown to be heterogeneous.

Keyword(s): cloning , cytomegalovirus , genome and mapping
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-59-1-111
1982-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/59/1/JV0590010111.html?itemId=/content/journal/jgv/10.1099/0022-1317-59-1-111&mimeType=html&fmt=ahah

References

  1. Birnboim H. C., Doly Y. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Clements J. B., Cortini R., Wilkie N. M. 1976; Analysis of herpesvirus DNA structure by means of restriction endonucleases. Journal of General Virology 30:243–256
    [Google Scholar]
  3. Downing R. G., Duggleby C. J., Villems R., Broda P. 1979; An endonuclease map of plasmid pWW0–8, a derivative of the TOL plasmid of Pseudomonas putida mt-2. Molecular and General Genetics 168:97–99
    [Google Scholar]
  4. Geder L., Sanford E. J., Rohner T. J., Rapp F. 1977; CMV and cancer of the prostate: in vitro transformation of human cells. Cancer Treatment Reports 61:139–146
    [Google Scholar]
  5. Geelen J. L. M. C., Walig C., Wertheim P., Van Der Noordaa J. 1978; Human cytomegalovirus DNA. 1.Molecular weight and infectivity. Journal of Virology 26:813–816
    [Google Scholar]
  6. Grunstein M., Hogness D. S. 1975; Colony hybridisation: a method for the isolation of cloned DNA’s that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of America 72:3961–3965
    [Google Scholar]
  7. Hanshaw J. B., Dudgeon J. A. 1978 Viral Diseases of the Foetus and Newborn Philadelphia: W. B. Saunders;
    [Google Scholar]
  8. Kushner R. S. 1978; An improved method for transformation of Escherichia coli with ColEI derived plasmids. In Genetic Engineering pp 17–23 Edited by Boyer H. W., Nicosia S. Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  9. Melnick J. L., Lewis R., Wimberley I., Kaufman R. H., Adam E. 1978; Association of CMV infection with cervical cancer: isolation of CMV from cell cultures derived from cervical biopsy. Intervirology 10:115–119
    [Google Scholar]
  10. Murray N. E., Bruce S. A., Murray K. 1979; Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product. Journal of Molecular Biology 132:493–505
    [Google Scholar]
  11. Nieman P. E., Reeves W., Ray G., Flournoy N., Lerner K. G., Sale G. E., Thomas E. D. 1977; A prospective analysis of interstitial pneumonia and opportunistic viral infection among recipients of allogenic bone marrow grafts. Journal of Infectious Diseases 136:754–767
    [Google Scholar]
  12. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  13. Roberts R. J. 1981; Restriction and modification enzymes and their recognition sequences. Nucleic Acids Research 9:75–96
    [Google Scholar]
  14. Roizman B. 1979; The organisation of the herpes simplex virus genomes. Annual Review of Genetics 13:25–57
    [Google Scholar]
  15. Rubin R. H., Cosimi A. B., Tokloff Rubin N. E. 1977; Infectious disease syndrome attributable to CMV and their significance among renal transplant recipients. Transplantation 24:458–464
    [Google Scholar]
  16. Skare J., Summers W. C. 1977; Structure and function of herpesvirus genomes. II. Eco RI, Xba I and Hin dIII endonuclease cleavage sites on herpes simplex type I DNA. Virology 76:581–595
    [Google Scholar]
  17. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  18. Stinski M. F., Mocarski E. S., Thomsen D. R. 1979; DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. Journal of Virology 31:231–239
    [Google Scholar]
  19. Weller T. H. 1971; The cytomegalovirus: ubiquitous agents with protein clinical manifestations. New England Journal of Medicine 285: 203–213 267–274
    [Google Scholar]
  20. Weststrate M. W., Geelen J. L. M. C., van Der Noordaa J. 1980; Human cytomegalovirus DNA: physical maps for the restriction endonucleases Bgl II, Hin dIII and Xba I. Journal of General Virology 49:1–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-59-1-111
Loading
/content/journal/jgv/10.1099/0022-1317-59-1-111
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error