1887

Abstract

SUMMARY

An indirect enzyme-linked immunosorbent assay (ELISA), using antibodies made against gradient-purified entomopoxvirus (EPV), detected down to 13 ng of virus protein. Little antigenic relatedness was detected by ELISA between the structural proteins of EPV, EPV, EPV and vaccinia virus. Antibodies made against EPV occlusion body matrix protein cross-reacted extensively with the occlusion body protein of EPV. A rapid increase in the biosynthesis of EPV structural proteins in (BTI-EAA) cells was detected from 12 to 50 h after virus infection. Low concentrations of virus-specific proteins were detected by ELISA in extracts of EPV-infected (Tn-368) cells from 1 to 96 h after virus inoculation.

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/content/journal/jgv/10.1099/0022-1317-57-1-215
1981-11-01
2024-04-26
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References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Analysis Biochemistry 72:248–254
    [Google Scholar]
  2. Cibulsky R. J., Happer J. D., Gudaukas R. T. 1977; Biochemical comparison of polyhedral protein from five nuclear polyhedrosis viruses infecting plusiine larvae (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 29:182–191
    [Google Scholar]
  3. Dales S., Mosbach E. H. 1968; Vaccinia as a model for membrane biogenesis. Virology 35:564–583
    [Google Scholar]
  4. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
    [Google Scholar]
  5. Garvey J. S., Cremer N. E., Sussdoref D. H. 1977; VI. Reagents. In Methods in Immunology pp 522–523 London: W. A. Benjamin;
    [Google Scholar]
  6. Krywienczyk J., Bergold G. H. 1961; Serological studies of inclusion-body proteins by agar diffusion technique. Journal of Insect Pathology 3:15–28
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Langridge W. H. R., Roberts D. W. 1977; Molecular weight of DNA from four entomopoxviruses determined by electron microscopy. Journal of Virology 21:301–308
    [Google Scholar]
  9. Mccarthy W. J., Granados R. R., Roberts D. E. 1974; Isolation and characterization of entomopoxvirus virions from virus-containing inclusions of Amsacta moorei (Lepidoptera: Arctiidae). Virology 21:301–308
    [Google Scholar]
  10. Mccarthy W. J., Neser C. F., Roberts D. W. 1975; RNA polymerase activity of Amsacta moorei entomopox virions. Intervirology 5:69–75
    [Google Scholar]
  11. Matthews R. E. F. 1979; Classification and nomenclature of viruses. Intervirology 12:160–164
    [Google Scholar]
  12. Pogo B. G. T., Dales S., Bergoin M., Roberts D. W. 1971; Enzymes associated with an insect poxvirus. Virology 43:306–309
    [Google Scholar]
  13. Roberts D. W., Bergoin M. 1970; Purification and characterization of an insect poxvirus of Lepidoptera. Proceedings of the International Colloquium of Insect Pathology College Park, Md., U.S.A 4:380–385
    [Google Scholar]
  14. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  15. Vaitukaitis J., Robbins B., Neischlag E., Ross G. T. 1971; A method for producing specific antisera with small doses of immunogen. Journal of Clinical Endocrinology 33:998–991
    [Google Scholar]
  16. Voller A., Bidwell D. E., Bartlett A. 1976; Enzyme immunoassays in diagnostic medicine. Bulletin of the World Health Organization 53:55–65
    [Google Scholar]
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