1887

Abstract

SUMMARY

An indirect enzyme-linked immunosorbent assay (ELISA), using antibodies made against gradient-purified entomopoxvirus (EPV), detected down to 13 ng of virus protein. Little antigenic relatedness was detected by ELISA between the structural proteins of EPV, EPV, EPV and vaccinia virus. Antibodies made against EPV occlusion body matrix protein cross-reacted extensively with the occlusion body protein of EPV. A rapid increase in the biosynthesis of EPV structural proteins in (BTI-EAA) cells was detected from 12 to 50 h after virus infection. Low concentrations of virus-specific proteins were detected by ELISA in extracts of EPV-infected (Tn-368) cells from 1 to 96 h after virus inoculation.

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/content/journal/jgv/10.1099/0022-1317-57-1-215
1981-11-01
2022-01-26
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