1887

Abstract

SUMMARY

Clover yellow mosaic virus (CYMV) protein encapsidates its RNA at pH 7.5, low ionic strength and at a temperature of 25 °C. Protected RNA fragments extracted from the initiation complexes contained the cap structure mGpppGp in the stoichiometric proportions expected for fragment sizes. These results were consistent with the location of the initiation site for assembly at or near the 5′ end of RNA; consequently, the maturation process is polar (5′ to 3′ direction). This strengthens the case for polar initiation as an alternative to internal initiation in the assembly of helical plant viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-57-1-199
1981-11-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/57/1/JV0570010199.html?itemId=/content/journal/jgv/10.1099/0022-1317-57-1-199&mimeType=html&fmt=ahah

References

  1. Abouhaidar M., Bancroft J. B. 1978a; The structure of the 5′ terminus of papaya mosaic virus RNA. Journal of General Virology 39:559–563
    [Google Scholar]
  2. Abouhaidar M., Bancroft J. B. 1978b; The initiation of papaya mosaic virus assembly. Virology 90:54–59
    [Google Scholar]
  3. Abouhaidar M., Bancroft J. B. 1979; Sequential encapsidation of heterologous RNA’s with papaya mosaic virus protein. Virology 93:253–255
    [Google Scholar]
  4. Abouhaidar M., Bancroft J. B. 1980; The polarity of assembly of papaya mosaic virus and tobacco mosaic virus RNA’s with PMV protein under conditions of non-specificity. Virology 107:202–207
    [Google Scholar]
  5. Abouhaidar M., Hirth L. 1977; 5′ terminal structure of tobacco rattle virus RNA: evidence for polarity of reconstitution. Virology 76:173–185
    [Google Scholar]
  6. Abouhaidar M., Erickson J. W., Bancroft J. B. 1979; The inhibition of papaya mosaic virus assembly related to the effect of cations on its RNA. Virology 98:116–120
    [Google Scholar]
  7. Bancroft J. B., Abouhaidar M., Erickson J. W. 1979; The assembly of clover yellow mosaic virus and its protein. Virology 98:121–130
    [Google Scholar]
  8. Butler P. J. G., Finch J. J., Zimmern D. 1977; Configuration of tobacco mosaic virus RNA during virus assembly. Nature, London 265:217–219
    [Google Scholar]
  9. Erickson J. W., Bancroft J. B. 1981; Melting of viral RNA by coat protein: assembly strategies for elongated plant viruses. Virology 108:235–240
    [Google Scholar]
  10. Lebeurier G., Nicolaieff A., Richards K. E. 1977; An inside-out model for the self-assembly of tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 74:149–153
    [Google Scholar]
  11. Zimmern D. 1977; The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA. Cell 11:463–482
    [Google Scholar]
  12. Zimmern D., Wilson T. M. A. 1976; Location of the origin for viral reassembly on tobacco mosaic virus RNA and its relation to stable fragment. FEBS Letters 71:294–298
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-57-1-199
Loading
/content/journal/jgv/10.1099/0022-1317-57-1-199
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error