The thermolability of transmissible gastroenteritis virus (TGEV) was studied between 31 and 55 °C using two different strains. The loss of infectivity followed first order kinetics except at the highest temperature. The values of the thermodynamic parameters indicated that the mechanisms involved above and below 45 °C are clearly distinct. The rates of inactivation were greater at alkaline than at neutral pH, yet the nature of the reaction appeared unchanged. Using four independent stocks of mutagenized virus, we failed to select thermal-resistant mutants by survivor selection at 38 °C. In contrast, thermal-resistant mutants were consistently obtained at 54 °C. However, the latter did not show any increased stability at 38 °C, confirming the fact that a different inactivation process takes place at high and at physiological temperatures.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error