1887

Abstract

SUMMARY

Lymphocytic choriomeningitis (LCM) virus interfering particles were enriched relative to infectious virions by ultracentrifugation in a shallow gradient made of Urografin. Electrophoretic analysis revealed that they lacked the small (‘S’) 23S RNA as well as GP-1 and GP-2 of the infectious virion and also lacked a newly characterized glycoprotein of apparent mol. wt. 85 × 10; instead, they contained a novel glycoprotein with mol. wt. 65 × 10.

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/content/journal/jgv/10.1099/0022-1317-55-2-475
1981-08-01
2022-01-25
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References

  1. Bonner W. M., Laskey R. Aa. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Buchmeier M. J., Oldstone M. B. A. 1979; Protein structure of lymphocytic choriomeningitis virus: evidence for a cell-associated precursor of the virion glycopeptides. Virology 99:111–120
    [Google Scholar]
  3. Buchmeier M. J., Elder J. H., Oldstone M. B. A. 1978; Protein structure of lymphocytic choriomeningitis virus: identification of the virus structural and cell associated polypeptides. Virology 89:133–145
    [Google Scholar]
  4. Gschwender H. H., Popescu M. 1976; Equilibrium sedimentation of virus in density gradients of iodinated compounds. In Biological Separations in Iodinated Density-Gradient Media pp 145–158 Edited by Rickwood D. London: Information Retrieval;
    [Google Scholar]
  5. Gschwender H. H., Brummund M., Lehmann-Grube F. 1975; Lymphocytic choriomeningitis virus. I. Concentration and purification of the infectious virus. Journal of Virology 15:1317–1322
    [Google Scholar]
  6. Harnish D., Dimock K., Leung W. C., Rawls W. 1981; Immunoprecipitable polypeptides in Pichinde virus infected BHK21 cells. In Replication of Negative Strand Viruses Edited by Bishop D. H. L., Compans R. W. New York: Elsevier/North-Holland; (in press)
    [Google Scholar]
  7. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10: pp 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Lehmann-Grube F. 1981; Infection of mice with lymphocytic choriomeningitis virus. In The Mouse in Biomedical Research Edited by Foster H. L., Small J. D., Fox J. G. New York: Academic Press; (in press)
    [Google Scholar]
  10. Lehmann-Grube F., Ambrassat J. 1977; A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. Journal of General Virology 37:85–92
    [Google Scholar]
  11. Lehmann-Grube F., Martinez Peralta L., Bruns M., Lohler J. 1981; Persistent infection of mice with lymphocytic choriomeningitis virus. In Comprehensive Virology vol 18: Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press; (in press)
    [Google Scholar]
  12. McMaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences of the United States of America 74:4835–4838
    [Google Scholar]
  13. Pedersen I. R. 1979; Structural components and replication of arenaviruses. Advances in Virus Research 24:277–330
    [Google Scholar]
  14. Popescu M., Lehmann-Grube F. 1976; Diversity of lymphocytic choriomeningitis virus: variation due to replication of the virus in the mouse. Journal of General Virology 30:113–122
    [Google Scholar]
  15. Popescu M., Lehmann-Grube F. 1977; Defective interfering particles in mice infected with lymphocytic choriomeningitis virus. Virology 77:78–83
    [Google Scholar]
  16. Popescu M., Schaefer H., Lehmann-Grube F. 1976; Homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference focus-forming units. Journal of Virology 20:1–8
    [Google Scholar]
  17. Rivers T. M., Scott T. F. M. 1936; Meningitis in man caused by a filterable virus. II. Identification of the etiological agent. Journal of Experimental Medicine 63:415–432
    [Google Scholar]
  18. Spaan W. J. M., Rottier P. J. M., Horzinek M. C., van der Zeijst B. A. M. 1981; Isolation and identification of virus-specific mRNAs in cells infected with mouse hepatitis virus (MHV-A59). Virology 108:424–434
    [Google Scholar]
  19. Traub E. 1939; Epidemiology of lymphocytic choriomeningitis in a mouse stock observed for four years. Journal of Experimental Medicine 69:801–817
    [Google Scholar]
  20. Welsh R. M., Buchmeier M. J. 1979; Protein analysis of defective interfering lymphocytic choriomeningitis virus and persistently infected cells. Virology 96:503–515
    [Google Scholar]
  21. Welsh R. M., Pfau C. J. 1972; Determinants of lymphocytic choriomeningitis interference. Journal of General Virology 14:177–187
    [Google Scholar]
  22. Welsh R. M., Burner P. A., Holland J. J., Oldstone M. B. A., Thompson H. A., Villarreal L. P. 1975; A comparison of biochemical and biological properties of standard and defective lymphocytic choriomeningitis virus. Bulletin of the World Health Organization 52:403–408
    [Google Scholar]
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