Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 55, Issue 2

Temperature-sensitive HVJ (Sendai Virus) with Altered P Polypeptide Derived from Persistently Infected Cell Lines No Access

Abstract

SUMMARY

HVJ isolated from culture fluids of G2, THEL and GM2 cells persistently infected with HVJ (G2-HVJ, THEL-HVJ and GM2-HVJ) were characterized in comparison with wild-type HVJ (HVJo). Viral structural proteins were analysed by 10% SDS-polyacrylamide gel electrophoresis and it was found that only the P polypeptides of all the HVJ clones isolated from G2-HVJ cells had a smaller size mol. wt. of 77000 (77K), than that of HVJo with a mol. wt. of 79000 (79K). One of six clones from THEL-HVJ cells and one of ten clones from GM2-HVJ cells exhibited the same migration pattern of P polypeptide as that of the clones from G2-HVJ cells. However, the other structural proteins were not different from those of the wild-type virions. All the clones from these carrier cultures were temperature-sensitive and were blocked in early step(s) required for RNA synthesis. These results indicate that some mutations(s) associated with P polypeptide could occur during the course of HVJ persistent infection in cell cultures.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): altered P protein , persistent infection , Sendai virus and ts mutant
© Journal of General Virology 1981
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-2-469
1981-08-01
2025-01-22
Loading full text...

Full text loading...

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
     [Google Scholar]
  2. Kimura Y. 1979; Tryptic peptide analysis of the structural proteins of a temperature-sensitive mutant derived from an HVJ (Sendai virus) carrier culture. Archives of Virology 61:157–161
     [Google Scholar]
  3. Kimura Y., Orvell C., Norrby E. 1979; Characterization of the polypeptides synthesized in cells infected with a temperature-sensitive mutant derived from HVJ (Sendai virus) carrier culture. Archives of Virology 61:23–33
     [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  5. Ogura H., Sato H., Tanaka J., Hatano M., Fukuda S., Morita O. 1980; Relationship between tumor formation and cell-mediated immunity in hamsters with transplanted HVJ (Sendai virus)-carrying tumor cells. Gann 71:325–332
     [Google Scholar]
  6. Portner A., Kingsbury D. W. 1972; Identification of transcriptive and replicative intermediates in Sendai virus-infected cells. Virology 47:711–725
     [Google Scholar]
  7. Raghow R., Kingsbury D. W. 1976; Endogenous viral enzymes involved in messenger RNA production. Annual Review of Microbiology 30:21–39
     [Google Scholar]
  8. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
     [Google Scholar]
  9. Sever J. L. 1962; Application of a microtechnique to viral serological investigations. Journal of Immunology 88:320–329
     [Google Scholar]
  10. Sugita K., Maru M., Sato K. 1974; A sensitive plaque assay for Sendai virus in an established line of monkey kidney cells. Japanese Journal of Microbiology 18:262–264
     [Google Scholar]
  11. Wechsler S. L., Rustlgian R., Stallcup K. C., Byers K. B., Winston S. H., Fields B. N. 1979; Measles virus-specified polypeptide synthesis in two persistently infected HeLa cell lines. Journal of Virology 31:677–684
     [Google Scholar]
  12. Yoshida T., Nagai Y., Maeno K., Iinuma M., Hamaguchi M., Matsumoto T., Nagayoshi S., Hoshino M. 1979; Studies on the role of M protein in virus assembly using a ts mutant of HVJ (Sendai virus). Virology 92:139–154
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-55-2-469
Loading
Temperature-sensitive HVJ (Sendai Virus) with Altered P Polypeptide Derived from Persistently Infected Cell Lines
J. Gen. Virol. 55, 469 (1981); https://doi.org/10.1099/0022-1317-55-2-469
/content/journal/jgv/10.1099/0022-1317-55-2-469
/content/journal/jgv/10.1099/0022-1317-55-2-469
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error