Three kinds of temperate phages were differentiated by serological, sedimentation and electron microscopic studies. Phage S1 had a long hexagonal head and a long tail, a density in CsSO of 1.365 g/ml and belonged to serological group A; phage S2 had a short hexagonal head and a short tail, a density of 1.385 to 1.392 g/ml and belonged to serological group B; phage S3 had a shape similar to phage S2 except for some minor differences, but had a density of 1.416 g/ml and belonged to serological group F. MS27 was found to be singly lysogenic for S2. However, MS3878 was a doubly lysogenic strain carrying S1 and S2 and E169 was a triply lysogenic strain carrying S1, S2 and S3. All these temperate phages showed similarity in shape to typing phages belonging to the same serological group. The temperate phages revealed the presence of a hexagonal baseplate with spikes. The burst sizes of phages S1, S2 and S3 were about 50, 110 and 120 respectively. The DNA from S1, S2 and S3 ranged from 29.4 to 30 megadaltons in size.


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