1887

Abstract

SUMMARY

‘A’ particles of Coxsackievirus B3 were generated from native virus by heating and purified by sucrose gradient centrifugation. These particles were found to be similar to ‘A’ particles formed by elution from cellular receptors of HeLa cells. Electrophoretic analysis of [S]methionine-labelled ‘A’ particles revealed that treatment of the particles with chymotrypsin resulted in the cleavage of VP1 and the formation of a cleavage product which migrated between VP2 and VP3. Analysis of the protease-treated material on sucrose gradients revealed a ribonuclease-sensitive particle which sedimented more slowly than an ‘A’ particle. This particle apparently degraded to release the viral RNA, thereby providing an model for protease-mediated uncoating of ‘A’ particles. The subviral particles of Coxsackievirus B3 were found to be immunoprecipitable with heterotypic Coxsackievirus group B antisera, thereby providing a method for the recovery of products produced in the cell early in infection. Infected cells which had been treated to remove unreacted virus were disrupted, and the lysates were reacted with heterotypic antisera. Analysis of the precipitated material revealed that no cleavage products were formed and no polypeptides were lost. Therefore, it appears that proteolysis is not involved in the uncoating of Coxsackievirus B3 in infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-2-439
1981-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/55/2/JV0550020439.html?itemId=/content/journal/jgv/10.1099/0022-1317-55-2-439&mimeType=html&fmt=ahah

References

  1. Baxt B., Bachrach H. L. 1980; Early interactions of Foot-and-Mouth disease with cultured cells. Virology 104:42–55
    [Google Scholar]
  2. Beatrice S. T., Katze M. G., Zajac B. A., Crowell R. L. 1980; Induction of neutralizing antibodies by the Coxsackievirus B3 virion polypeptide, VP2. Virology 104:426–438
    [Google Scholar]
  3. Beneke T. W., Habermehl K. O., Diefenthal W., Buchhol Z. M. 1977; Iodination of poliovirus Capsid proteins. Journal of General Virology 34:387–390
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Breindl M. 1971; The structure of heated poliovirus particles. Journal of General Virology 11:147–156
    [Google Scholar]
  6. Cavanagh D., Sanger D. V., Rowlands D. J., Brown F. 1977; Immunogenic and cell attachment sites of FMDV: further evidence for their location in a single capsid polypeptide. Journal of General Virology 35:149–158
    [Google Scholar]
  7. Cavanagh D., Rowlands D. J., Brown F. 1978; Early events in the interaction between foot-and-mouth disease virus and primary pig kidney cells. Journal of General Virology 41:255–264
    [Google Scholar]
  8. Crowell R. L., Philipson L. 1971; Specific alterations of Coxsackievirus B3 eluted from HeLa cells. Journal of Virology 8:509–515
    [Google Scholar]
  9. Crowell R. L., Siak J.-S. 1978; A receptor for the group B Coxsackieviruses: characterization and extraction from HeLa cell plasma membranes. Perspectives in Virology 10:39–55
    [Google Scholar]
  10. Crowell R. L., Landau B. J., Philipson L. 1971; The early interaction of Coxsackievirus B3 with HeLa cells. Proceedings of the Society for Experimental Biology and Medicine 137:1082–1088
    [Google Scholar]
  11. DeSena J., Mandel B. 1976; Studies on the in vitro uncoating of poliovirus. I. Characterization of the modifying factor and the modifying reaction. Virology 70:470–483
    [Google Scholar]
  12. DeSena J., Mandel B. 1977; Studies on the in vitro uncoating of poliovirus. II. Characteristics of the membrane-modified particle. Virology 78:554–556
    [Google Scholar]
  13. DeSena J., Torian B. 1980; Studies on the in vitro uncoating of poliovirus. III. Roles of membrane modifying and stabilizing factors in the generation of subviral particles. Virology 104:149–163
    [Google Scholar]
  14. Fenwick M. L., Cooper P. D. 1962; Early interactions between poliovirus and ERK cells: some observations on the nature and significance of the rejected particles. Virology 18:212–223
    [Google Scholar]
  15. Fenwick M. L., Wall M. J. 1973; Factors determining the site of synthesis of poliovirus proteins: the early attachment of virus particles to endoplasmic membranes. Journal of Cellular Sciences 13:403–413
    [Google Scholar]
  16. Guttman N., Baltimore D. 1977; A plasma membrane component able to bind and alter virions of poliovirus type 1: studies on a cell-free alteration using a simplified assay. Virology 82:25–36
    [Google Scholar]
  17. Holland J. J., Hoyer B. H. 1962; Early stages of enterovirus infection. Cold Spring Harbor Symposia on Quantitative Biology 23:101–111
    [Google Scholar]
  18. Jokuk W. K., Darnell J. E. 1961; The adsorption and early fate of purified poliovirus in HeLa cells. Virology 13:439–447
    [Google Scholar]
  19. Katze M. G., Crowell R. L. 1980; Immunological Studies of the group B Coxsackieviruses by the sandwich Enzyme-linked Immunosorbent Assay (ELISA) and immunoprecipitation. Journal of General Virology 50:357–367
    [Google Scholar]
  20. Lonberg-Holm K., Butterworth B. 1976; Investigation of the structure of polio- and human rhinoviruses through the use of selective chemical reactivity. Virology 71:207–216
    [Google Scholar]
  21. Lonberg-Holm K., Philipson L. 1974; Early interactions between animal viruses and cells. Monographs in Virology 9:1–148
    [Google Scholar]
  22. Lonberg-Holm K., Gosser L. B., Kauer J. C. 1975; Early alteration of poliovirus in infected cells and its specific inhibition. Journal of General Virology 27:329–342
    [Google Scholar]
  23. Lonberg-Holm K., Gosser L. B., Shimshick E. J. 1976; Interaction of liposomes with subviral particles of poliovirus type 2 and rhinovirus type 2. Journal of Virology 19:746–749
    [Google Scholar]
  24. McGeady M. L. 1980 Characterization of Coxsackievirus B3 ‘A’ particles, a structural intermediate produced during the early events of virus-cell interaction Ph.D. thesis Hahnemann Medical College; Philadelphia:
    [Google Scholar]
  25. McGeady M. L., Crowell R. L. 1979; Stabilization of‘A’ particles of Coxsackievirus B3 by a HeLa cell plasma membrane extract. Journal of Virology 32:790–795
    [Google Scholar]
  26. Maizel J. V. 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:176–246
    [Google Scholar]
  27. Philipson L., Beatrice S. T., Crowell R. L. 1973; A structural model for picornaviruses as suggested from an analysis of urea degraded virions and procapsids of Coxsackievirus B3. Virology 54:69–79
    [Google Scholar]
  28. Roesing T. G., Toselli P. A., Crowell R. L. 1975; Elution and uncoating of Coxsackievirus B3 by isolated HeLa cell plasma membranes. Journal of Virology 15:654–667
    [Google Scholar]
  29. Rosenwirth B., Eggers H. J. 1979; Early process of echovirus 12-infection: elution, penetration and uncoating under the influence of rhodanine. Virology 97:241–255
    [Google Scholar]
  30. Siak J.-S., McGeady M. L., Crowell R. L. 1978 In Abstracts of the IV International Congress on Virology p 182
    [Google Scholar]
  31. Wild T. F., Brown F. 1967; Nature of the inactivating action of trypsin on foot-and-mouth disease virus. Journal of General Virology 1:247–250
    [Google Scholar]
  32. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
  33. Zajac I., Crowell R. L. 1969; Differential inhibition of attachment and eclipse activities of HeLa cells for enteroviruses. Journal of Virology 3:422–428
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-2-439
Loading
/content/journal/jgv/10.1099/0022-1317-55-2-439
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error