S1 nuclease analysis and 3′ terminal sequencing of the reovirus genome double-stranded RNAs and produced viral mRNAs has been used to establish that the viral mRNA is a full length copy of the genome template. Sequence determination at the 3′ end of the genome minus strand has by transposition allowed determination of the 5′ terminal sequences of the viral mRNAs. In all but one case an AUG codon which could function in initiation of protein synthesis has been found within the determined sequence. The 3′ ends of the plus strands from all genome segments were found to have a common sequence. The implications of these results on the mechanism of virus replication are discussed.

Keyword(s): dsRNA , mRNA , reovirus and sequencing

Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error