1887

Abstract

SUMMARY

Suppressive effects of human IFN-α, IFN- and mouse-IFN on syncytium formation in human and mouse transformed cells have been studied using u.v.-irradiated Sendai virus (UV-Sendai virus). After treatment of human RSa cells with 500 units/ml human IFN-α for 16 h, the syncytium formation induced by UV-Sendai virus was reduced to less than 10% of controls. The suppressive effect was dependent on the amount of interferon added, and it was blocked by the addition of cycloheximide. Suppression of syncytium formation was also observed on human IF cells, which are partially resistant to the anticellular effect of interferon but are sensitive to the antiviral effect of interferon. Human IFN- also had a suppressive effect on syncytium formation in human transformed cells, and human IFN-α, IFN- and mouse IFN showed species specificity in their effect on fusion. This anti-cell fusion activity was developed in IF cells from about 5 h after addition of IFN-α and when the cells were treated with IFN-α for 16 h, the resistant state of cells continued for over 20 h after removal of IFN-α.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-2-289
1981-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/55/2/JV0550020289.html?itemId=/content/journal/jgv/10.1099/0022-1317-55-2-289&mimeType=html&fmt=ahah

References

  1. Besançon F., Ankel H. 1978; Membrane receptors for interferon. Texas Reports on Biology and Medicine 35:283–292
    [Google Scholar]
  2. Brouty-Boye D., Zetter B. R. 1980; Inhibition of cell motility by interferon. Science 208:516–518
    [Google Scholar]
  3. Cantell K., Hirvonen S. 1977; Preparation of human leukocyte interferon for clinical use. Texas Reports on Biology and Medicine 35:138–144
    [Google Scholar]
  4. Chang E. H., Jay F. T., Friedman R. M. 1978; Physical, morphological, and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proceedings of the National Academy of Sciences of the United States of America 75:1859–1863
    [Google Scholar]
  5. Chatterjee S., Hunter E. 1980; Inhibition of Mason-Pfizer monkey virus-induced syncytium formation in normal human cells by homologous interferon. Virology 104:487–490
    [Google Scholar]
  6. Chen H. Y., Sato T., Fuse A., Kuwata T., Content J. 1981; Resistance to interferon of a human adenocarcinoma cell line, HEC-1, and its sensitivity to natural killer cell action. Journal of General Virology 52:177–181
    [Google Scholar]
  7. Edy V. G., van Damme J., Billiau A., De Somer P. 1978; Human interferon: large scale production in embryo fibroblast cultures. In Human Interferon and Clinical Use pp 55–60 Edited by Stinebring W. R., Chappie P. J. New York: Plenum Press;
    [Google Scholar]
  8. Friedman M. R. 1979; Interferons: interactions with cell surfaces. In Interferon 1: pp 53–74 Edited by Gresser I. New York and London: Academic Press;
    [Google Scholar]
  9. Gresser I. 1977; On the varied biological effects of interferon. Cellular Immunology 34:406–415
    [Google Scholar]
  10. Holmgren J., Svennerholm L., Elwing H., Fredman P., Strannegard O. 1980; Sendai virus receptor: proposed recognition structure based on binding to plastic-adsorbed gangliosides. Proceedings of the National Academy of Sciences of the United States of America 77:1947–1950
    [Google Scholar]
  11. Hosaka Y., Shimizu K. 1977; Cell fusion by Sendai virus. In Virus Infection and Cell Surface pp 129–155 Edited by Poste G., Nicolson G. L. Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  12. Knight E. JG, Korant B. D. 1977; A cell surface alteration in mouse L cells induced by interferon. Biochemical and Biophysical Research Communications 74:707–713
    [Google Scholar]
  13. Kohn L. D., Friedman M. R., Holmes M. J., Lee G. 1976; Use of thyrotropin and chorela toxin to probe the mechanism by which interferon initiates its antiviral activity. Proceedings of the National Academy of Sciences of the United States of America 73:3695–3699
    [Google Scholar]
  14. Kuramoto H., Tamura S., Notake Y. 1972; Establishment of a cell line of human endometrial adenocarcinoma in vitro. American Journal of Obstetrics and Gynecology 15:1012–1019
    [Google Scholar]
  15. Kuwata T., Fuse A., Morinaga N. 1976; Effect of interferon on cel! and virus growth in transformed human cell lines. Journal of General Virology 33:7–15
    [Google Scholar]
  16. Kuwata T., Fuse A., Morinaga N. 1977; Effects of cycloheximide and puromycin on the antiviral and anticellular activities of human interferon. Journal of General Virology 37:195–198
    [Google Scholar]
  17. Kuwata T., Fuse A., Suzuki N., Morinaga N. 1978; Comparison of the suppression of cell and virus growth in transformed human cells by leukocyte and fibroblast interferon. Journal of General Virology 43:435–439
    [Google Scholar]
  18. Lindahl P., Leary P., Gresser I. 1973; Enhancement by interferon of the expression of surface antigens on murine leukemia L 1210 cells. Proceedings of the National Academy of Sciences of the United States of America 70:2785–2788
    [Google Scholar]
  19. Lindahl P., Gresser I., Leary P., Tovey M. 1976; Interferon treatment of mice: enhanced expression of histocompatibility antigens on lymphoid cells. Proceedings of the National Academy of Sciences of the United States of America 73:1284–1287
    [Google Scholar]
  20. Matsuyama M. 1979; Action of interferon on cell membrane of mouse lymphocytes. Experimental Cell Research 124:253–259
    [Google Scholar]
  21. Okada Y., Murayama F. 1966; Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ. Experimental Cell Research 44:527–551
    [Google Scholar]
  22. Pfeffer L. M., Wang E., Tamm I. 1980a; Interferon inhibits the redistribution of cell surface components. Journal of Experimental Medicine 152:469–474
    [Google Scholar]
  23. Pfeffer L. M., Wang E., Tamm I. 1980b; Interferon effects on microfilament organization, cellular fibronectin. distribution, and cell motility in human fibroblasts. Journal of Cell Biology 85:9–17
    [Google Scholar]
  24. Poste G., Pasternak C. A. 1978; Virus induced cell fusion. In Membrane and Fusion pp 306–367 Edited by Poste G., Nicolson G. L. Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  25. Sato T., Tomita Y., Kuwata T. 1980; Inhibition by interferon of Sendai virus cytolysis. Infection and Immunity 28:6–10
    [Google Scholar]
  26. Stewart W. E. II (editor) 1979 The Interferon System Wien and New York: Springer-Verlag;
    [Google Scholar]
  27. Stewart W. E. II, De Clercq E., Billiau A., Desmyter J., De Somer P. 1972; Increased susceptibility of cells treated with interferon to the toxicity of poly-riboinosinic. polyribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
    [Google Scholar]
  28. Tomita Y., Kuwata T. 1979; Suppressive effects of interferon on syncytium formation by RD-114 virus in human transformed cells. Journal of General Virology 43:111–117
    [Google Scholar]
  29. Tomita Y., Kuwata T. 1980; Interferon inhibits cell fusion by Sendai virus. Annals of the New York Academy of Sciences 350:625
    [Google Scholar]
  30. Vandenbussche P., Divizia M., Verhaegen M., Fuse A., Kuwata T., De Clercq E., Content J. 1981; Enzymatic activities induced by interferon in human fibroblast cell lines differing in their sensitivity to the anticellular activity of interferon. Virology (in press)
    [Google Scholar]
  31. Verhaegen M., Divizia M., Vandenbussche P., Kuwata T., Content J. 1980; Abnormal behaviour of interferon-induced enzymatic activities in an interferon-resistant cell line. Proceedings of the National Academy of Sciences of the United States of America 77:4479–4483
    [Google Scholar]
  32. Vignaux F., Gresser I. 1977; Differential effects of interferon on the expression of H-2K, H-2D and la antigens on mouse lymphocytes. Journal of Immunology 118:721–723
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-2-289
Loading
/content/journal/jgv/10.1099/0022-1317-55-2-289
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error