1887

Abstract

Summary

Pichinde virus preparations were investigated for ribosomal components and associated activities. After detergent treatment and fractionation, viral components were assayed for polypeptide synthesis, elongation of nascent polypeptide chains, and mRNA activity. It was demonstrated that these subviral particles could synthesize polypeptides when exogenous mRNA template, aminoacyl-tRNAs, translation factors, GTP and appropriate cations were added. Undisrupted, whole virions could not synthesize polypeptide; disruption and subsequent centrifugation of subviral ribosomes was a prerequisite for the biosynthetic activity described.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-1-213
1981-07-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/55/1/JV0550010213.html?itemId=/content/journal/jgv/10.1099/0022-1317-55-1-213&mimeType=html&fmt=ahah

References

  1. Carter M. F., Biswal N., Rawls W. E. 1972; Characterization of nucleic acid of Pichinde virus. Journal of Virology 11:61–68
    [Google Scholar]
  2. Farber F. E., Rawls W. E. 1975; Isolation of ribosome-like structures from Pichinde virus. Journal of General Virology 26:21–31
    [Google Scholar]
  3. Floyd R. S., Stone M. P., Joklik W. K. 1974; Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Analytical Biochemistry 59:599–609
    [Google Scholar]
  4. Gangemi J. D., Rosato R. R., Connell E. V., Johnson E. M., Eddy G. A. 1978; Structural polypeptides of Machupo virus. Journal of General Virology 41:183–188
    [Google Scholar]
  5. Haralson M. A., Roufa D. J. 1975; A temperature-sensitive mutation affecting the mammalian 60S ribosome. Journal of Biological Chemistry 250:8618–8623
    [Google Scholar]
  6. Leung W. C., Rawls W. E. 1977; Virion-associated ribosomes are not required for the replication of Pichinde virus. Virology 81:174–176
    [Google Scholar]
  7. Mannweiler K., Lehmann-grube F. 1973; Electron microscopy of LCM virus-infected L cell. In Lymphocytic Choriomeningitis Virus and other Arenaviruses pp 37–38 Edited by Lehmann-Grube F. Berlin: Springer-Verlag;
    [Google Scholar]
  8. Moldave K., Sadnik I. 1979; Preparation of derived and native ribosomal subunits from rat liver. Methods in Enzymology 59:402–410
    [Google Scholar]
  9. Moldave K., Galasinski W., Rao P. 1971; Polypeptide chain elongation factors from rat liver. Methods in Enzymology 20:337–348
    [Google Scholar]
  10. Murphy F. A., Webb P. A., Johnson K. M., Whitfield S. G., Chappell W. 1970; Arenaviruses in Vero cells: ultrastructural studies. Journal of Virology 6:507–518
    [Google Scholar]
  11. Pedersen I. R. 1971; Lymphocytic choriomeningitis virus RNAs. Nature New Biology 234:112–114
    [Google Scholar]
  12. Pedersen I. R. 1973; Different classes of ribonucleic acid isolated from lymphocytic choriomeningitis virus. Journal of Virology 11:416–423
    [Google Scholar]
  13. Pedersen I. R., Knigshofer E. P. 1976; Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus. Journal of Virology 20:14–21
    [Google Scholar]
  14. Sadnik I., Herrera F., Mccuiston J., Thompson H. A., Moldave K. 1975; Studies on native ribosomal subunits from rat liver. Evidence for activities associated with native 40S subunits that affect the interaction with acetylphenylalanyl-tRNA, methionyl-tRNAp and 60S subunits. Biochemistry 14:5328–5335
    [Google Scholar]
  15. Siler J., Moldave K. 1969; Studies on the binding of phenylalanyl transfer RNA to rat-liver ribosomes. Biochimica etBiophysica Acta 195:123–129
    [Google Scholar]
  16. Skogerson L., Moldave K. 1968; Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reaction. Archives of Biochemistry and Biophysics 125:497–505
    [Google Scholar]
  17. Vezza A. C., Gard G. P., Compans R. W., Bishop D. H. L. 1977; Structural components of the arenavirus Pichinde. Journal of Virology 23:776–786
    [Google Scholar]
  18. Vezza A. C., Clewley J. P., Gard G. P., Abraham N. Z., Compans R. W., Bishop D. H. L. 1978; Virion RNA species of the arenaviruses Pichinde, Tacaribe, and Tamiami. Journal of Virology 26:485–497
    [Google Scholar]
  19. Welsh R. M., Burner P. A., Holland J. J., Oldstone M. B. A., Thompson H. A., Villarreal L. P. 1975; A comparison of biochemical and biological properties of standard and defective lymphocytic choriomeningitis virus. Bulletin of the World Health Organization 52:403–408
    [Google Scholar]
  20. Yamane T., Sueoka N. 1963; Conservation of specificity between amino acid acceptor RNA and aminoacyl-sRNA synthetase. Proceedings of the National Academy of Sciences of the United States of America 50:1093–1100
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-1-213
Loading
/content/journal/jgv/10.1099/0022-1317-55-1-213
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error