1887

Abstract

Summary

Previous investigation of the ability of cytomegalovirus and varicella-zoster virus to replicate in a variety of cell lines suggested that both virus types plaqued with high efficiency in mink lung cells. However, many of the virus isolates used appeared to be contaminated with mycoplasma. We now report that the observed cytopathic effect is due to a mycoplasma which grows lytically to high titre in mink lung cells, but is difficult to cultivate in cell-free media. The mycoplasma was plaque-purified and shown to contain DNA with a buoyant density of 1.684 g/ml, with restriction endonuclease patterns identical to the porcine mycoplasma . This was confirmed by serological identification.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-55-1-201
1981-07-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/55/1/JV0550010201.html?itemId=/content/journal/jgv/10.1099/0022-1317-55-1-201&mimeType=html&fmt=ahah

References

  1. Barile M. F. 1979; Mycoplasma-tissue cell interactions. In The Mycoplasmas vol 2 pp 425–474 Edited by Tully J. G., Whitcomb R. F. New York: Academic Press;
    [Google Scholar]
  2. Darai G., Flügel R. M. 1978; Mink lung cells: a non-primate cell line highly susceptible for varicella-zoster virus. Journal of General Virology 41:179–181
    [Google Scholar]
  3. Darai O., Flügel R. M. 1979; Erratum. Journal of General Virology 42:655
    [Google Scholar]
  4. Davis R. W., Simon M., Davidson N. 1968; Electron microscopy heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  5. Freundt E. A. 1975; The mycoplasmas. In Bergey’s Manual of Determinative Bacteriology pp 929–958 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  6. Gelderblom H., Ogura H., Bauer H. 1974; On the occurrence of oncornavirus-like particles in HeLa cells. Cytobiology 8:339–344
    [Google Scholar]
  7. Goodwin R. F. W., Pomeroy A., Whittlestone P. 1967; Characterization of mycoplasma suipneumoniae: mycoplasma causing enzootic pneumonia of pigs. Journal of Hygiene 65:85–91
    [Google Scholar]
  8. Gross Bellard M., Oudet P., Chambon P. 1973; Isolation of high-molecular weight DNA from mammalian cells. European Journal of Biochemistry 36:32–38
    [Google Scholar]
  9. Leach R. H., Butler M. 1966; Comparison of mycoplasmas associated with human tumors, leukemia, and tissue cultures. Journal of Bacteriology 91:934–941
    [Google Scholar]
  10. Sheldrick P., Laithier M., Lando D., Ryhiner M. L. 1973; Infectious DNA from herpes simplex virus: infectivity of double-stranded and single-stranded molecules. Proceedings of the National Academy of Sciences of the United States of America 70:3621–3625
    [Google Scholar]
  11. Tegtmeyer P., Macasaet F. 1972; Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis. Journal of Virology 10:599–604
    [Google Scholar]
  12. Teplitz M. 1977; Isolation of folded chromosome from mycoplasma hyorhinis. Nucleic Acids Research 4:1505–1512
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-55-1-201
Loading
/content/journal/jgv/10.1099/0022-1317-55-1-201
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error