Carrier cultures of L cells infected with wild-type vesicular stomatitis virus (VSV) were initiated without the use of defective-interfering particles or homologous interferon. The cloned viruses recovered from such carrier cultures after passage 21 were characterized as temperature-sensitive. Furthermore, these clones of the mutant showed restricted replication at permissive temperature in HEp-2 cell cultures as compared to the wild-type VSV. This restrictive replication of the mutant in HEp-2 cells was not due to a defect in the expression of virion-associated primary transcriptase activity , but due to the marked reduction in virus-specific amplified RNA synthesis. These results suggest that a host function(s) yet to be characterized may govern the synthesis of mutant virus-specific amplified RNA in HEp-2 cells.


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