1887

Abstract

Summary

Carrier cultures of L cells infected with wild-type vesicular stomatitis virus (VSV) were initiated without the use of defective-interfering particles or homologous interferon. The cloned viruses recovered from such carrier cultures after passage 21 were characterized as temperature-sensitive. Furthermore, these clones of the mutant showed restricted replication at permissive temperature in HEp-2 cell cultures as compared to the wild-type VSV. This restrictive replication of the mutant in HEp-2 cells was not due to a defect in the expression of virion-associated primary transcriptase activity , but due to the marked reduction in virus-specific amplified RNA synthesis. These results suggest that a host function(s) yet to be characterized may govern the synthesis of mutant virus-specific amplified RNA in HEp-2 cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-2-449
1981-06-01
2019-10-24
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-54-2-449
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error