1887

Abstract

SUMMARY

The distribution of guanine-cytosine (GC) pairs in the DNA of the highly oncogenic simian adenovirus type 7 (SA7) and the non-oncogenic human adenovirus type 6 (Ad6) has been studied by thermal denaturation and CsCl density-gradient centrifugation. The differential of the DNA thermal denaturation curves shows the presence of pronounced peaks which indicates uneven distribution of GC pairs along the DNA chains and the presence of regions with GC content from 30 to 74% in SA7 DNA and from 40 to 68% in Ad6 DNA. The DNA restriction fragments obtained by treatment with RI, HI, I, II and dIII were subjected to CsCl density-gradient centrifugation. GC content of the fragments ranged from 45 to 70% for SA7 DNA and from 43 to 61% for Ad6 DNA. The GC content of the extreme left-hand fragments, where the transforming gene(s) is located, was higher than the average for SA7 DNA and lower than the average for Ad6 DNA. The most GC-rich regions were localized in the centre of the genome. The GC content of the right-hand part of both viral genomes was lower than the average.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-2-425
1981-06-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/54/2/JV0540020425.html?itemId=/content/journal/jgv/10.1099/0022-1317-54-2-425&mimeType=html&fmt=ahah

References

  1. Bachenheimer S., Bellet A. J. D., Darnel J. E., Doerfler W., Van Der EB A. J., Flint S. J., Fujinaga K., Gesteland R. F., Ginsberg H. S., Green M., Horwitz M. S., Kelly T. S., Levin A. S., Levin A. J., Lewis A. M., Mak S., Mcdougall J. K., Pearson G. D., Pettersson U., Philipson L., Raska K., Raskas H. J., Roberts R., Rose J. A., Russell W. C., Sambrook J., Schlesinger R. W., Sharp P. A., Shimojo H., Sussenbach J. S., Tibbetts C., Van Der Vliet P. C., Weber J., Winnacker E. L., Wold W. S. M. 1977; Adenovirus standard nomenclature: a proposal. Journal of Virology 22:830–831
    [Google Scholar]
  2. De Ley J. 1970; Reexamination of association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738–754
    [Google Scholar]
  3. Dukema R., Dekker B. M. M., Van Ormondt H. 1980; The nucleotide sequence of the transforming Bgl II-H fragment of adenovirus type 7 DNA. Gene 9:141–156
    [Google Scholar]
  4. Dimitrov D. H., DubitcheV A. G., Naroditsky B. S., Dreizin R. S., Tikchonenko T. I. 1979; Physicochemical properties and restriction maps of simian adenovirus type 38 DNA. Journal of General Virology 44:68–80
    [Google Scholar]
  5. Dubichev A. G., Parfenov N. N., Chaplygina N. M., Borovik A. S., Dreizin R. S., Zolotarskaya E. E., Tikchonenko T. I. 1978; Physico-chemical properties of human adenovirus type 6 DNA. Voprosi viruso-logii 1:47–51
    [Google Scholar]
  6. Erikson R. I., Szybalski W. 1964; The Cs2S04 equilibrium density gradient and its application for the study of T-even phage DNA: glucosylation and replication. Virology 22:111–124
    [Google Scholar]
  7. Goodheart C. R. 1971; DNA density of oncogenic and nononcogenic simian adenoviruses. Virology 44:645–648
    [Google Scholar]
  8. Lyubchenko YU. L., FrankKamenetski M. D., Vologodski A. V., Lasurkin YU. S., Gause G. G. 1976; Fine structure of DNA melting curves. Biopolymers 15:1019–1036
    [Google Scholar]
  9. Naroditsky B. S., Zavizion B. A., Karamov E. V., Chaplygina N. M., Dreizin R. S., Zolotarskaya E. E., Tikchonenko T. I. 1978; Fragmentation of simian adenovirus type 7 DNA by specific endonucleases R.Sall and R.BamHI. Voprosi virusologii 1:36–42
    [Google Scholar]
  10. Naroditsky B. S., Kalinina T. I., Golberg E. Z., Borovik A. S., Karamov E. V., Tikchonenko T. I. 1980; Analysis of DNA from human adenovirus type 6 with restriction endonucleases Hind III, Bgl II and Bam HI. Biochimica et Biophysica Acta 606:214–217
    [Google Scholar]
  11. Pavlov V. M., Lyubchenko YU. L., Borobik A. S., Lasurkin YU. S. 1977; Specific fragmentatio of T7 phage DNA at low-melting sites. Nucleic Acids Research 4:4053–4062
    [Google Scholar]
  12. Ponomareva T. I., Grodnitskaya N. A., Goldberg N. M., Chaplygina E. Z., Naroditski B. S., Tikchonenko T. I. 1979; Biological activity of the intact cleavage DNA of simian adenovirus 7. Nucleic Acids Research 6:3119–3131
    [Google Scholar]
  13. Pina M., Green M. 1965; Biochemical studies on adenovirus multiplication. Proceedings of the National Academy of Sciences of the United States of America 54:547–551
    [Google Scholar]
  14. Sugisaki H., Sugimoto-Shiroki K., Saito I., Vemizu Y., Vesugi S. 1980; Structure and gene organization in the transforming Hindlll-G fragment of AdI2. Cell 20:547–551
    [Google Scholar]
  15. Tikchonenko T. I., Dreizin R. S., Kalinina T. I., Ryvkina M. B., Zolotarskaya E. E. 1980; Analysis of Ad6 DNA by the restrictases Hpal and Smal and localization of oncogene. In Viruses of Cancer and Leucosis pp 12–14 Edited by Zhdanov V. M., Tikchonenko T. I. Moscow: Academy of Medical Sciences of the USSR;
    [Google Scholar]
  16. Van Ormondt H., Maat J., De Waard A., Van Der EB A. J. 1978; The nucleotide sequence of the transforming Hpal E fragment of adenovirus type 5 DNA. Gene 4:309–328
    [Google Scholar]
  17. Zavizion B. A., Goldberg E. Z., Naroditsky B. S., Zolotarskaya E. D., Tikchonenko T. I. 1979; Fragmentation of simian adenovirus type 7 (SA7) DNA with specific endonucleases R.Bglll and R.Hindlll. Voprosi virusologii 6:595–599
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-54-2-425
Loading
/content/journal/jgv/10.1099/0022-1317-54-2-425
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error