1887

Abstract

SUMMARY

The murine coronavirus strains MHV JHM, MHV 1, MHV 2, MHV 3 and MHV A59 were tested for their neurovirulence in weanling rats. The strain JHM was found to be highly neurovirulent for weanling rats, whereas the other strains were not, or only slightly, neurovirulent. MHV 1 caused no lesions in weanling rats. The other strains (MHV 2, MHV 3 and MHV A59) induced predominantly subclinical infections in weanling rats as demonstrated by an increase of antibodies and inflammatory lesions in the liver. Analysis of these strains by cross-neutralization revealed variable degrees of antigenic relationship between these viruses which were not related to their neurovirulence. However, when these strains were compared by analysing the T-RNase-resistant oligonucleotides of virion RNA, the highly neurovirulent strain JHM was found to differ significantly in its nucleotide sequence from the other less-neurovirulent strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-54-1-67
1981-05-01
2022-09-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/54/1/JV0540010067.html?itemId=/content/journal/jgv/10.1099/0022-1317-54-1-67&mimeType=html&fmt=ahah

References

  1. Bailey O. T., Pappenheimer A. M., Cheever F., Daniels J. B. 1949; A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. Journal of Experimental Medicine 90:195–212
    [Google Scholar]
  2. Cheever F. S., Daniels I. B., Pappenheimer A. M., Bailey O. T. 1949; A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. I. Isolation and biological properties of the virus. Journal of Experimental Medicine 90:181–194
    [Google Scholar]
  3. Clewley J. P., Bishop D. H. L., Kang C. Y., Coffin J., Schnitzlein W. M., Reichmann M. E., Shope R. E. 1977; Oligonucleotide fingerprints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup. Journal of Virology 23:152–166
    [Google Scholar]
  4. De Wachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  5. Dick G. W. A., Niven J. F. S., Gledhill A. W. 1956; A virus related to that causing hepatitis in mice (MHV). British Journal of Experimental Pathology 37:90–97
    [Google Scholar]
  6. Gledhill A. W., Andrewes C. A. 1951; A hepatitis virus of mice. British Journal of Experimental Pathology 32:559–568
    [Google Scholar]
  7. Hierholzer J. C., Broderson J. R., Murphy F. A. 1979; New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infection and Immunity 24:508–522
    [Google Scholar]
  8. Holland J. J., Grabau E. A., Jones C. L., Semler B. L. 1979; Evolution of multiple genome mutations during long-term persistent infection by vesicular stomatitis virus. Cell 16:95–504
    [Google Scholar]
  9. Lai M. M. C., Stohlman S. A. 1978; RNA of mouse hepatitis virus. Journal of Virology 26:236–242
    [Google Scholar]
  10. Lampert P. W., Sims J. K., Kniazeff A. J. 1973; Mechanism of demyelination in JHM virus encephalomyelitis. Electron microscopic studies. Acta Neuropathologica 24:6–85
    [Google Scholar]
  11. Manaker R. A., Piczak C. V., Miller A. A., Stanton M. F. 1961; A hepatitis virus complicating studies with mouse leukemia. Journal of the National Cancer Institute 27:29–44
    [Google Scholar]
  12. Nagashima K., Wege H., Ter Meulen V. 1978a; Early and late CNS-effects of coronavirus infection in rats. In Advances in Experimental Medicine and Biology vol 100: pp 395–409 Edited by Palo J. New York: Plenum Press;
    [Google Scholar]
  13. Nagashima K., Wege H., Meyermann R., Ter Meulen V. 1978b; Coronavirus induced subacute demyelinating encephalomyelitis in rats. A morphological analysis. Acta Neuropathologica 44:63–70
    [Google Scholar]
  14. Nagashima K., Wege H., Meyermann R., Ter Meulen V. 1979; Demyelinating encephalomyelitis induced by a long-term coronavirus infection in rats. Acta Neuropathologica 45:205–213
    [Google Scholar]
  15. Nelson J. B. 1952; Acute hepatitis associated with mouse leukemia. I. Pathological features and transmission of the disease. Journal of Experimental Medicine 96:293–303
    [Google Scholar]
  16. Roy P., Clewley J. P. 1978; Spring viremia of carp virus RNA and virion-associated transcriptase activity. Journal of Virology 25:912–916
    [Google Scholar]
  17. Siddell S. G., Wege H., Barthel A., Ter Meulen V. 1980; Coronavirus JHM: cell-free synthesis of structural protein p. 60. Journal of Virology 33:10–17
    [Google Scholar]
  18. Stephenson J. R., Hay A. J., Skehel J. 1977; Characterization of virus specific messenger RNAs from avian fibroblasts infected with fowl plague virus. Journal of General Virology 36:237–248
    [Google Scholar]
  19. Trent D., Grant J. A. 1980; A comparison of new world alphaviruses in the western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques. Journal of General Virology 47:261–282
    [Google Scholar]
  20. Wege H., Muller A., Ter Meulen V. 1978; Genomic RNA of the murine coronavirus JHM. Journal of General Virology 41:217–227
    [Google Scholar]
  21. Weiland E., Mussgay M., Weiland F. 1978; Nonproducer malignant tumor cells with rescuable sarcoma virus genome isolated from a recurrent Moloney sarcoma. Journal of Experimental Medicine 148:08–423
    [Google Scholar]
  22. Weiner L. P. 1973; Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus). Archives of Neurology 28:293–303
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-54-1-67
Loading
/content/journal/jgv/10.1099/0022-1317-54-1-67
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error