1887

Abstract

SUMMARY

The sensitivity of mouse mammary tumour virus (MMTV) and murine leukaemia virus (MuLV) to interferon was tested by investigating the effect of interferon in cultures of mouse mammary tumour cells simultaneously synthesizing the two viruses. The dose—response curves showed that the extracellular production of both viruses by these cells was equally inhibited by interferon treatment. In contrast, the intracellular steady-state concentration of virus-specific RNA of both MMTV and MuLV was not significantly affected in these cells. This was the case regardless of whether total cellular RNA or polysomal poly(A)-containing RNA was examined. These results are consistent with the suggestion that interferon-caused inhibition is related to a host-mediated effect on a post-transcriptional step in virus synthesis.

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1981-04-01
2022-01-24
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References

  1. Allen P. T., Schellekens H., Van Griensven L. J. L. D., Billiau A. 1976; Differential sensitivity of Rauscher murine leukemia virus (MuLV-R) to interferons in two interferon-responsive cell lines. Journal of General Virology 31:429–435
    [Google Scholar]
  2. Arya S. K. 1980a; Phorbol ester-mediated stimulation of the synthesis of mouse mammary tumor virus. Nature, London 284:71–72
    [Google Scholar]
  3. Arya S. K. 1980b; Changes in retrovirus expression in mouse mammary tumor cells in culture. Cancer Research (in press)
    [Google Scholar]
  4. Arya S. K., Young N. A. 1980; Synthesis in high yield of complementary DNA of retrovirus RNA. Preparative Biochemistry 10:483–493
    [Google Scholar]
  5. Arya S. K., Gordon B., Young N. A. 1979; Polynucleotide inhibition of RNA directed DNA polymerase of type B (MMTV) and type C (SiSV) retroviruses. Molecular Pharmacology 16:297–305
    [Google Scholar]
  6. Arya S. K., Czarniecki C. W., Friedman R. M. 1980; Effect of interferon on the synthesis of mouse mammary tumor virus RNA and proteins. Journal of Interferon Research 1:147–154
    [Google Scholar]
  7. Billiau A., Edy V. G., Sobis H., De somer P. 1974; Influence of interferon on virus particle synthesis in oncornavirus-carrier lines. II. Evidence for a direct effect on particle release. International Journal of Cancer 14:335–340
    [Google Scholar]
  8. Billiau A., Edy V. G., De clercq E., Heremans H., Desomer P. 1975; Influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. III. Survey of effects on A-, B- and C-type oncornaviruses. International Journal of Cancer 15:947–953
    [Google Scholar]
  9. Chirgwin J. M., Pryzbyla A. E., Macdonald R. J., Rutter W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  10. Dion A. S., Vaidya A. B., Fout G. S. 1974; Cation preference for poly (rC).oligo (dG)-directed synthesis of RNA tumor viruses and human milk particulates. Cancer Research 34:3509–3515
    [Google Scholar]
  11. Fan H., Macissac P. 1978; Virus-specific RNA synthesis in interferon-treated mouse cells productively infected with Moloney murine leukemia virus. Journal of Virology 27:449–452
    [Google Scholar]
  12. Friedman R. M., Chang E. H., Ramseur J. M., Meyers M. W. 1975; Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers. Journal of Virology 16:569–574
    [Google Scholar]
  13. Glisin V., Crvenjakav R., Byus C. 1974; Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633–2637
    [Google Scholar]
  14. Pitha P., Rowe W. P., Oxman M. N. 1976; Effect of interferon on exogenous, endogenous and chronic murine leukemia virus infection. Virology 70:324–338
    [Google Scholar]
  15. Sala-trepat J. M., Savage M. J., Bonner J. 1978; Isolation and characterization of poly (adenylic acid)-containing messenger ribonucleic acid from rat liver polysomes. Blochimica et Biophvsica Acta 519:173–193
    [Google Scholar]
  16. Schidlovsky G., Shibley G. P., Benton C. V., Elser J. E. 1978; Type B and type C RNA tumor virus replication in single cells. Electron microscopy with tannic acid. Journal of the National Cancer Institute 61:91–95
    [Google Scholar]
  17. Schochetman G., Fine D. L., Massey R. J. 1978; Mouse mammary tumor virus and murine leukemia virus cell surface antigens on virus producer and non-producer mammary epithelial tumor cells. Virology 88:379–383
    [Google Scholar]
  18. Sen G. C., Sarkar N. H. 1980; Effects of interferon on the production of murine mammary tumor virus by mammary tumor cells in culture. Virology 102:431–443
    [Google Scholar]
  19. Stewart W. E. II 1979 Interferon System pp 230–250 New York: Springer-Verlag;
    [Google Scholar]
  20. Strauchen J. A., Young N. A., Friedman R. M. 1977; Interferon mediated inhibition of mouse mammary tumor virus expression in cultured cells. Virology 82:232–236
    [Google Scholar]
  21. Strauchen J. A., Ary A S. K., Tralka T. S., Engel L. W., Young N. A. 1980; Search for retrovirus-like particles in human breast cancer cells in culture. Cancer Research 40:3880–3885
    [Google Scholar]
  22. Yagi M. J., King N. W. Jr, Bekesi J. G. 1980; Alterations of a mouse mammary tumor virus glycoprotein with interferon treatment. Journal of Virology 34:225–233
    [Google Scholar]
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