1887

Abstract

Summary

The early genes of bacteriophage T2 are partially excluded from the progeny of crosses between the related bacteriophages T2 and T4. This is due to complete exclusion from the progeny of six exclusion-sensitive sites in T2. A mutation [(56)1] in the sensitive site near T2 gene 56 renders the site partially resistant against exclusion.

This paper describes the mapping of the (56)1 mutation. The mutation was mapped between two clusters of amber 56 mutations in T2, but mapping was not completely unequivocal. Additional evidence for location of (56)1 within gene 56 was provided by the decrease in the activity of the gene 56 product (dCTPase: EC 3.6.1.12) induced by T2 (56)1 strains. The location of (56)1 within an essential gene contradicts the exclusion model proposed by Russell & Huskey (1974).

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1981-03-01
2022-01-28
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References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Hattman S. 1964; The functioning of T-even phages with unglucosylated DNA in restricting Escherichia coli host cells. Virology 24:333–348
    [Google Scholar]
  3. Kim J-S., Davidson N. 1974; Electron microscope heteroduplex study of the sequence relation of T2, T4 and T6 bacteriophage DNAs. Virology 57:93–111
    [Google Scholar]
  4. Mahmood N., Lunt M. R. 1972; Biochemical changes during mixed infections with bacteriophages T2 and T4. Journal of General Virology 16:185–197
    [Google Scholar]
  5. Masamune Y. 1968; The effect of ultraviolet light on the intracellular stability of the DNA of bacteriophages T2 and T4. Molecular and General Genetics 101:99–110
    [Google Scholar]
  6. Miller J. H., Ganem D., Lu P., Schmitz A. 1977; Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map. Journal of Molecular Biology 109:275–301
    [Google Scholar]
  7. O’Farrell P. Z., Gold L. M., Huang W. M. 1973; The identification of prereplicative bacteriophage T4 proteins. Journal of Biological Chemistry 248:5499–5501
    [Google Scholar]
  8. Okker R. J. H. 1974; The fate of parental DNA of bacteriophage T2 in crosses with bacteriophage T4. Biochimica et Biophysica Acta 353:36–44
    [Google Scholar]
  9. Okker R. J. H., De Groot B. 1978; Specificity of partial exclusion of bacteriophage T2 in crosses with T4. Journal of General Virology 40:705–709
    [Google Scholar]
  10. Pees E. 1970; Bacteriophage T4 mutants unable to exclude gene 56 of T2 from the progeny of crosses. Mutation Research 9:345–348
    [Google Scholar]
  11. Pees E., De Groot B. 1970; Partial exclusion of genes of bacteriophage T2 with T4-glucosylated DNA in crosses with bacteriophage T4. Genetica 41:541–550
    [Google Scholar]
  12. Pees E., De Groot B. 1975; Mutants of bacteriophage T4 unable to exclude T2 from the progeny of crosses. Virology 67:94–106
    [Google Scholar]
  13. Price A. R., Warner H. R. 1969; Bacteriophage T4-induced deoxycytidine triphosphate-deoxyuridine triphosphate nucleotidohydrolase: its properties and its role during phage infection of Escherichia coli. Virology 37:882–892
    [Google Scholar]
  14. Revel H. R., Georgopoulos C. P. 1969; Restriction of non-glucosylated T-even bacteriophages by prophage PI. Virology 39:1–17
    [Google Scholar]
  15. Revel H. R., Hattman S., Lurja S. E. 1965; Mutants of bacteriophage T2 and T6 defective in α-glucosyltransferase. Biochemical and Biophysical Research Communications 18:545–550
    [Google Scholar]
  16. Russell R. L. 1974; Comparative genetics of the T-even bacteriophages. Genetics 78:967–988
    [Google Scholar]
  17. Russell R. L., Huskey R. J. 1974; Partial exclusion between T-even bacteriophages: an incipient genetic isolation mechanism. Genetics 78:989–1014
    [Google Scholar]
  18. Stahl F. W., Edgar R. S., Steinberg J. 1964; The linkage map of bacteriophage T4. Genetics 50:539–552
    [Google Scholar]
  19. Wiberg I. S., Mendelsohn S., Warner V., Hercules K., Aldrich C., Munro J. L. 1973; SP62, a viable mutant of bacteriophage T4D defective in regulation of phage enzyme synthesis. Journal of Virology 12:775–792
    [Google Scholar]
  20. Wood W. B., Revel H. R. 1976; The genome of bacteriophage. T4 Bacteriological Reviews 40:847–868
    [Google Scholar]
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