1887

Abstract

SUMMARY

Coronavirus JHM contains six major proteins, one of which, the 60000 mol. wt. nucleocapsid protein pp60, is phosphorylated. In JHM-infected cells ip 60K, the intracellular precursor to pp60 is also phosphorylated. Associated with purified JHM virions is a protein kinase which will phosphorylate pp60 and a variety of exogenous substrates . The enzyme has the characteristics of a cyclic nucleotide-independent protein kinase. Both the reaction and the enzyme activity transferred the γ-phosphate of ATP to serine residues on the nucleocapsid protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-52-2-235
1981-02-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/52/2/JV0520020235.html?itemId=/content/journal/jgv/10.1099/0022-1317-52-2-235&mimeType=html&fmt=ahah

References

  1. Blair G. E., Russell W. C. 1978; Identification of a protein kinase activity associated with human adenoviruses. Virology 86:157–166
    [Google Scholar]
  2. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  3. Lai M. M. C., Stohlman S. A. 1978; RNA of mouse hepatitis virus. Journal of Virology 26:236–248
    [Google Scholar]
  4. Lamb R. A. 1975; The phosphorylation of Sendai virus proteins by a virus particle-associated protein kinase. Journal of General Virology 26:249–263
    [Google Scholar]
  5. Mcintosh K. 1974; Coronaviruses: a comparative review. Current Topics in Microbiology and Immunology 63:85–129
    [Google Scholar]
  6. Nagashima K., Wege H., Meyermann R., Ter Meulen V. 1978; Coronavirus induced subacute demyelinating encephalitis in rats: a morphological analysis. Acta Neuropathologica 44:63–70
    [Google Scholar]
  7. Nagashima K., Wege H., Meyermann R., Ter Meulen V. 1979; Demyelinating encephalomyelitis induced by a long term coronavirus infection in rats. Acta Neuropathologica 45:205–213
    [Google Scholar]
  8. O’farrell P. Z., Goodman H. M., O’farrell P. H. 1977; High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142
    [Google Scholar]
  9. Robb R. A., Bond C. W. 1980; Coronaviridae. In Comprehensive Viro vol 14: pp 195–247 Edited by FraenkelConrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  10. Siddell S. G., Wege H., Barthel A., Ter Meulen V. 1980; Coronavirus JHM: cell-free synthesis of structural protein p60. Journal of Virology 33:10–17
    [Google Scholar]
  11. Smith A. E., Wheeler T., Glanville N., Kaariainen L. 1976; Translation of Semliki Forest virus 42S RNA in a mouse cell-free system to give virus coat protein. European Journal of Biochemistry 49:101–110
    [Google Scholar]
  12. Stohlman S. A., Lai M. M. C. 1979; Phosphoproteins of murine hepatitis viruses. Journal of Virology 32:672–675
    [Google Scholar]
  13. Sturman L. S., Holmes K. V., Behnke J. 1980; Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. Journal of Virology 33:449–462
    [Google Scholar]
  14. Tan K. B. 1975; Comparative study of the protein kinase associated with animal viruses. Virology 64:566–570
    [Google Scholar]
  15. Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., MacNaughton M. R., Mcintosh K. 1978; Coronaviridae: second report. Intervirology 10:321–328
    [Google Scholar]
  16. Wege H., Muller A., Ter Meulen V. 1978; Genomic RNA of the murine coronavirus JHM. Journal of General Virology 41:217–227
    [Google Scholar]
  17. Wege H., Wege H., Nagashima K., Ter Meulen V. 1979; Structural polypeptides of the murine coronavirus JHM. Journal of General Virology 42:37–47
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-52-2-235
Loading
/content/journal/jgv/10.1099/0022-1317-52-2-235
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error