1887

Abstract

SUMMARY

Syrian golden hamster interferon was made by stimulating secondary or benzo(α)pyrene-transformed embryo cells with Newcastle disease virus. Titres of 1000 to 6000 units/ml of tissue culture fluid were obtained. The ionic properties of this interferon were characterized by chromatography on cation and anion exchangers and also by isoelectric focusing when two components were observed: a major component, pI 5.8 and a minor component, pI 6.6. Hamster interferon was partially purified on a tandem of sorbents of diverse chromatographic bias: anion exchanger → metal chelate → hydrophobic ligand. The purified preparation had a specific activity of about 1 × 10 units/mg protein; the recovery of activity was nearly complete. The apparent mol. wt. of hamster interferon, as estimated by SDS-polyacrylamide gel electrophoresis under non-reducing conditions, is 17000. Crude Syrian hamster interferon preparations had some activity on mouse cells; this heterologous activity was entirely due to a small subpopulation of interferon molecules which could be isolated on phenylagarose.

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1981-02-01
2024-11-03
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References

  1. Berman L. D., Sato E. 1978; Comparison of spontaneous soft tissue hamster tumors with those induced by viral and chemical carcinogens. Federation Proceedings 37:2089–2090
    [Google Scholar]
  2. Bohlen P., Stein S., Dairman W., Udenfriend S. 1973; Fluorometric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
    [Google Scholar]
  3. Bollin E. Jr, Sulkowski E. 1978; Metal chelate affinity chromatography of hamster interferon. Archives of Virology 58:149–152
    [Google Scholar]
  4. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  5. Gresser I., Bandu M. T., Brouty-Boye D., Tovey M. 1974; Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature, London 251:543–545
    [Google Scholar]
  6. Havell E. A., Hayes T. G., Vilcek J. 1978; Synthesis of two distinct interferons by human fibroblasts. Abstracts of the Annual Meeting of the A merican Society for Microbiology246
    [Google Scholar]
  7. Klein J. 1978; Syrian hamsters: do they or don’t they have an MHC?. Federation Proceedings 37:2061–2062
    [Google Scholar]
  8. Maizel J. V. JR 1966; Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science 151:988–990
    [Google Scholar]
  9. Ng M. H., Vilcek J. 1972; Interferons: physicochemical properties and control of cellular synthesis. Advances in Protein Chemistry 26:173–241
    [Google Scholar]
  10. Porath J., Carlsson J., Olsson I., Belfrage G. 1975; Metal chelate affinity chromatography, a new approach to protein fractionation. Nature, London 258:598–599
    [Google Scholar]
  11. Radola B. J. 1973; Isoelectric focusing in layers of granulated gels. I. Thin-layer isoelectric focusing of proteins. Biochimica et Biophysica Acta 295:412–428
    [Google Scholar]
  12. Stewart W. E. II, Declercq E., De Somer P. 1974; Stabilization of interferons by defensive reversible denaturation. Nature, London 249:460–461
    [Google Scholar]
  13. Streilein J. W. 1978; Hamster immune responses: experimental models linking immunogenetics, oncogenesis and viral immunity. Federation Proceedings 37:2023–2024
    [Google Scholar]
  14. Sulkowski E., Bollin E. JR, Jankowski W. J., Carter W. A. 1977; Tandem affinity chromatography of hamster and mouse interferons. Federation Proceedings 36:741
    [Google Scholar]
  15. Talas M., Weiszfeiler G., Batkai L. 1968; Hamster interferons induced by polyoma virus. Acta Virologica 12:378–380
    [Google Scholar]
  16. Toolan H. W. 1978; Susceptibility of the Syrian hamster to virus infection. Federation Proceedings 37:2065–2069
    [Google Scholar]
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