1887

Abstract

Summary

Syrian golden hamster interferon was made by stimulating secondary or benzo(α)pyrene-transformed embryo cells with Newcastle disease virus. Titres of 1000 to 6000 units/ml of tissue culture fluid were obtained. The ionic properties of this interferon were characterized by chromatography on cation and anion exchangers and also by isoelectric focusing when two components were observed: a major component, pI 5.8 and a minor component, pI 6.6. Hamster interferon was partially purified on a tandem of sorbents of diverse chromatographic bias: anion exchanger → metal chelate → hydrophobic ligand. The purified preparation had a specific activity of about 1 × 10 units/mg protein; the recovery of activity was nearly complete. The apparent mol. wt. of hamster interferon, as estimated by SDS-polyacrylamide gel electrophoresis under non-reducing conditions, is 17000. Crude Syrian hamster interferon preparations had some activity on mouse cells; this heterologous activity was entirely due to a small subpopulation of interferon molecules which could be isolated on phenylagarose.

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/content/journal/jgv/10.1099/0022-1317-52-2-227
1981-02-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-52-2-227
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