1887

Abstract

SUMMARY

Two kinds of hepatitis B virus-specific particles are present in cytoplasmic extracts of hepatoma cells synthesizing hepatitis B virus (HBV) surface antigen. One class of particle contains the surface antigen of the virus, is 20S in size and has a buoyant density (in CsCl) of 1.2 g/ml. The second class of particle is a deoxyribonucleoprotein (DNP) with 1.3 g/ml buoyant density (in CsCl) and is 30S in size, the DNA of which contains HBV sequences thus proving virus specificity.

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/content/journal/jgv/10.1099/0022-1317-51-2-341
1980-12-01
2021-10-19
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References

  1. Alexander J., Macnab G., Saunders R. 1978; Studies on in vitro production of hepatitis B surface antigen by a human cell line. Perspectives in Virology 10:103–117
    [Google Scholar]
  2. Baltimore D., Huang A. 1968; Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science 162:572–574
    [Google Scholar]
  3. Burrell C. J., Leadbetter G., Mackay P., Marmion B. P. 1976; Tryptic cleavage of antibody binding sites from hepatitis B surface antigen particles. Journal of General Virology 33:41–50
    [Google Scholar]
  4. Burrell C. J., Mackay P., Greenway P. J., Hofschneider P. H., Murray K. 1979; Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR 322. Nature, London 279:43–47
    [Google Scholar]
  5. Charnay P., Pourcel C., Louise A., Fritsch A., Tiollais P. 1979; Cloning in Escherichia coli and physical structure of hepatitis B virion DNA. Proceedings of the National Academy of Sciences of the United States of America 75:2222–2226
    [Google Scholar]
  6. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charney P. 1979; Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature, London 281:646–650
    [Google Scholar]
  7. Delap R. J., Rush M. G., Zouzigas D., Khan S. 1978; Isolation and preliminary characterization of small circular DNA present in African green monkey kidney (BSC-1) cells. Plasmid 1:508–521
    [Google Scholar]
  8. Hofschneider P. H., Zaslavsky V., Deinhardt F., Frösner G. G., Alexander J. 1979; Labeling Of hepatitis B virus surface antigen (HBsAg) synthesized in a HBsAg-producing hepatoma cell line. Journal of Medical Virology 4:171–175
    [Google Scholar]
  9. Kaplan P. M., Greenman R. L., Gerin J. L., Purcell R. H., Robinson W. S. 1973; DNA polymerase associated with human hepatitis B antigen. Journal of Virology 12:995–1005
    [Google Scholar]
  10. Ling C. M., Overby L. R. 1972; Prevalence of hepatitis B virus antigen as revealed by direct radioimmunoassay with 125I antibody. Journal of Immunology 109:834–841
    [Google Scholar]
  11. Marion P. L., Robinson W. S. 1980; Hepatitis B virus in a human hepatocellular carcinoma cell line. In Viruses in Naturally Occuring Cancer Edited by Essex M., Todaro G., zur Hausen H. Cold Spring Harbor Conference on Cell Proliferation vol 7: (in press)
    [Google Scholar]
  12. Pasek M., Goto T., Gilbert W., Zink B., Scaller H., Mackay P., Leadbetter G., Murray K. 1979; Hepatitis B virus genes and their expression in E. coll. Nature, London 282:575–579
    [Google Scholar]
  13. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling of deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  14. Robinson W. S. 1977; The genome of hepatitis B virus. Annual Review of Microbiology 31:357–377
    [Google Scholar]
  15. Shinsky J. J., Siddiqui A., Robinson W. S., Cohen S. 1979; Cloning and endonuclease mapping of the hepatitis B viral genome. Nature, London 279:346–348
    [Google Scholar]
  16. Spirin A. S., Belitsina N.V., Lerman M. 1965; Use of formaldehyde fixation for studies of ribonucleo-protein particles by caesium chloride density gradient centrifugation. Journal of Molecular Biology 14:611–615
    [Google Scholar]
  17. Valenzuela P., Gray P., Quiroga M., Zaldivar J., Goodman H. M., Rutter W. J. 1979; Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature, London 280:815–819
    [Google Scholar]
  18. Verma I. M. 1978; Genome organization of RNA tumor viruses. 1. In vitro synthesis of full genome length single-stranded and double-stranded viral DNA transcripts. Journal of Virology 26:615–629
    [Google Scholar]
  19. Vyas G. N., Williams E. W., Klaus G. G. B., Bond H. E. 1972; Hepatitis-associated Australian antigen. Protein, peptides, and amino acid composition of purified antigen with its use in determining sensitivity of the hemagglutination test. Journal of Immunology 108:1114–1118
    [Google Scholar]
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