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1887

Journal of General Virology header logo Journal of General Virology

Volume 51, Issue 2

Replication of RNA-1 of Tomato Black Ring Virus Independently of RNA-2 No Access

Abstract

SUMMARY

In hybridization experiments, using complementary DNA (cDNA) copies of the two genome parts of tomato black ring virus (TBRV RNA-1 and RNA-2), no sequence homology between the two RNA species was detected.

When tobacco mesophyll protoplasts were inoculated with purified middle component particles, which contain only RNA-2, no replication of TBRV RNA could be detected. However, when they were inoculated with purified bottom component particles, which contain only RNA-1, extracts made 24 or 48 h later contained RNA that had the same mobility as RNA-1 in polyacrylamide-agarose gels, and that hybridized to cDNA copies of RNA-1. Thus RNA-1 can replicate in protoplasts that do not contain RNA-2. Moreover, this RNA-1 was capable, when mixed with nucleoprotein particles containing RNA-2, of inducing the formation of local lesions in leaves, and therefore was intact and attached to the genome-linked protein. The genome-linked protein of nepoviruses is probably virus-coded, and its production in protoplasts inoculated with bottom component particles therefore suggests that RNA-1 contains the gene for this protein.

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© Journal of General Virology 1980
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1980-12-01
2025-11-14

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References

  1. Bruening G. 1977; Plant covirus systems: two-component systems. In Comprehensive Virology vol 11: pp 55–141 Edited by Fraenkel-Conrat H., Wagner R. R. New York and London: Plenum Press;
     [Google Scholar]
  2. Bruening G., Agrawal H. O. 1967; Infectivity of a mixture of cowpea mosaic virus ribonucleoprotein components. Virology 32:306–320
     [Google Scholar]
  3. Cadman C. H. 1962; Evidence for association of tobacco rattle virus nucleic acid with a cell component. Nature, London 193:49–52
     [Google Scholar]
  4. Daubert S. D., Bruening G., Najarian R. C. 1978; Protein bound to the genome RNAs of cowpea mosaic virus. European Journal of Biochemistry 92:45–51
     [Google Scholar]
  5. El Manna M. M., Bruening G. 1973; Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology 56:198–206
     [Google Scholar]
  6. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
     [Google Scholar]
  7. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses. Virology 88:361–370
     [Google Scholar]
  8. Gould A. R., Symons R. H. 1977; Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4:3787–3802
     [Google Scholar]
  9. Hanada K., Harrison B. D. 1977; Effects of virus genotype and temperature on seed transmission of nepoviruses. Annals of Applied Biology 85:79–92
     [Google Scholar]
  10. Harrison B. D., Barker H. 1978; Protease-sensitive structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
     [Google Scholar]
  11. Harrison B. D., Murant A. F. 1977; Nematode transmissibility of pseudo-recombinant isolates of tomato black ring virus. Annals of Applied Biology 86:209–212
     [Google Scholar]
  12. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569–581
     [Google Scholar]
  13. Harrison B. D., Murant A. F., Mayo M. A. 1972a; Evidence for two functional RNA species in raspberry ringspot virus. Journal of General Virology 16:339–348
     [Google Scholar]
  14. Harrison B. D., Murant A. F., Mayo M. A. 1972b; Two properties of raspberry ringspot virus determined by its smaller RNA. Journal of General Virology 17:137–141
     [Google Scholar]
  15. Harrison B. D., Murant A. F., Mayo M. A., Roberts I. M. 1974; Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virus. Journal of General Virology 22:233–247
     [Google Scholar]
  16. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
     [Google Scholar]
  17. Kubo S., Harrison B. D., Barker H. 1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
     [Google Scholar]
  18. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
     [Google Scholar]
  19. Maxwell I. H., Van Ness J., Hahn W. E. 1978; Assay of DNA-RNA hybrids by S1nuclease digestion and adsorption to DEAE-cellulose filters. Nucleic Acids Research 5:2033–2038
     [Google Scholar]
  20. Mayo M. A., Barker H. 1980; Protein linked to nepovirus RNA. Annual Report, Scottish Horticultural Research Institute1979 (in the press)
    [Google Scholar]
  21. Mayo M. A., Barker H., Harrison B. D. 1979a; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
     [Google Scholar]
  22. Mayo M. A., Barker H., Harrison B. D. 1979b; Evidence for a protein covalently linked to tobacco ringspot virus RNA. Journal of General Virology 43:735–740
     [Google Scholar]
  23. Murant A. F., Taylor M., Duncan O. H. 1979; Estimates of molecular weight of virus RNA by electrophoresis and by electron microscopy. Annual Report, Scottish Horticultural Research Institute 1978: pp 98–99
     [Google Scholar]
  24. Randles J. W., Harrison B. D., Murant A. F., Mayo M. A. 1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
     [Google Scholar]
  25. Rezaian M. A., Francki R. I. B. 1974; Replication of tobacco ringspot virus. II. Differences in nucleotide sequences between the viral RNA components. Virology 59:275–280
     [Google Scholar]
  26. Rottier P. 1980; Viral protein synthesis in cowpea mosaic virus infected protoplasts. Mededelingen Land-bouwhoogeschool Wageningen 80: No 3 96 pp
    [Google Scholar]
  27. Rottier P. J. M., Rezelman G., Van Kammen A. 1980; Protein synthesis in cowpea mosaic virus-infected cowpea protoplasts: further characterization of virus-related proteins. Journal of General Virology 51:373–383
     [Google Scholar]
  28. Sänger H. L., Brandenburg E. 1961; Über die Gewiiinung von infektiosem Presssaft aus ‘Wintertyp’-Pflanzen des Tabak-Rattle-Virus durch Phenolextraktion. Naturwissenschaften 48:391–393
     [Google Scholar]
  29. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. 1978; A protein linked to the 5′ termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Research 5:4505–4522
     [Google Scholar]
  30. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et Biophysica Acta 442:324–330
     [Google Scholar]
  31. van Kammen A. 1968; The relationship between the components of cowpea mosaic virus. I. Two ribo-nucleoprotein particles necessary for the infectivity of CPMV. Virology 34:312–318
     [Google Scholar]
  32. Vogt V. M. 1973; Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. European Journal of Biochemistry 33:192–200
     [Google Scholar]
  33. Wood H. A., Bancroft J. B. 1965; Activation of a plant virus by related incomplete nucleoprotein particles. Virology 27:94–102
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-51-2-317
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Replication of RNA-1 of Tomato Black Ring Virus Independently of RNA-2
J. Gen. Virol. 51, 317 (1980); https://doi.org/10.1099/0022-1317-51-2-317
/content/journal/jgv/10.1099/0022-1317-51-2-317
/content/journal/jgv/10.1099/0022-1317-51-2-317
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