1887

Abstract

SUMMARY

Two strains of fixed rabies virus were examined for their ability to regenerate defective interfering (DI) particles and for possible correlation of DI particle production with the expression of virulence. A plaque-purified stock of the attenuated ERA strain (ERA), which characteristically caused an auto-interfering death response in adult mice inoculated i.c., was serially passed at a high m.o.i. in BHK-21 cells. By the sixth passage, DI particles were regenerated that corresponded in sedimentation velocity and DI/RNA size to the smallest of three sizes of DI particles produced by the parental stock virus. The regeneration of ERA DI particles was not detected during 15 serial high or low m.o.i. passages of infected newborn mouse brain, though the passaged virus consistently elicited an auto-interfering-type death response when assayed in adult mice. The attenuated Flury HEP strain regenerated up to three unique size classes of DI particles during serial passage in BHK-21 or murine neuroblastoma C1300 clone NA cells compared with the one band of DI particles produced by the parental Flury HEP stock virus. The BHK-21 cell-adapted Flury HEP virus failed to kill adult mice when inoculated at high concentrations after two serial passages in NA cells. However, the virus became fully virulent and a single band of regenerated DI particles was visible. Additional bands of defective particles were visible following the third serial passage in NA cells. Single-stranded RNA with a mol. wt. of 0.62 × 10 was extracted from the first DI particle population to be regenerated. This corresponded in mol. wt. to the DI/ssRNA characteristic of the parental attenuated Flury HEP virus. However, in the parental type DI/RNA, partially dsRNA could be isolated in addition to ssRNA. Double-stranded RNA could not be detected in the regenerated DI particles derived from the virulent NA cell-propagated Flury HEP virus. These results suggest that the virulence phenotype of fixed rabies viruses does not depend on the presence or absence of DI particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-51-1-69
1980-11-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/51/1/JV0510010069.html?itemId=/content/journal/jgv/10.1099/0022-1317-51-1-69&mimeType=html&fmt=ahah

References

  1. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gel. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  2. Clark H F. 1978; Rabies viruses increase in virulence when propagated in neuroblastoma cell culture. Science 199:1072–1075
    [Google Scholar]
  3. Clark H F. 1979; Factors affecting the virulence for mice of fixed rabies viruses. Proceedings of the 4th Munich Symposium on Microbiology on Mechanisms of Viral Pathogenesis and Virulence pp 281–300 Edited by Bachman P. A.
    [Google Scholar]
  4. Clark H F., Parks N. F., Wunner W. H. 1980; Defective interfering particles of fixed rabies viruses; lack of correlation with attenuated or auto-interfering patterns of mouse-pathogenicity. Journal of General Virology 52: (in press)
    [Google Scholar]
  5. Crick J., Brown F. 1974; An interfering component of rabies virus which contains RNA. Journal of General Virology 22:147–151
    [Google Scholar]
  6. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  7. Holland J. J., Villarreal L. P., Breindl M. 1976; Factors involved in the generation and replication of rhabdovirus defective T particles. Journal of Virology 17:805–815
    [Google Scholar]
  8. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  9. Kawai A., Matsumoto S., Tanabe K. 1975; Characterisation of rabies viruses recovered from persistently infected BHK cells. Virology 67:520–533
    [Google Scholar]
  10. Koprowski H. 1954; Biological modification of rabies virus as a result of its adaptation to chicks and developing chick embryos. Bulletin of the World Health Organization 10:709–724
    [Google Scholar]
  11. Koprowski H., Black J., Nelsen D. J. 1954; Studies on chick-embryo-adapted rabies virus. VI. Further changes in pathogenic properties following prolonged cultivation in the developing chick embryo. Journal of Immunology 72:94–106
    [Google Scholar]
  12. Lazzarini R. A., Weber G. H., Johnson L. D., Stamminger G. M. 1975; Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. Journal of Molecular Biology 97:289–307
    [Google Scholar]
  13. Macpherson L., Stoker M. 1962; Polyoma transformation of hamster cell clones – an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  14. Sedwick W. D., Wiktor T. J. 1967; Reproducible plaquing system for rabies, lymphocytic choriomeningitis, and other ribonucleic acid viruses in BHK-21/13S agarose suspensions. Journal of Virology 1:1224–1226
    [Google Scholar]
  15. Sokol F., Clark H F. 1973; Phosphoproteins, structural components of rhabdoviruses. Virology 52:246–263
    [Google Scholar]
  16. Wiktor T. J., Dietzschold B., Leamnson R. N., Koprowski H. 1977; Induction and biological properties of defective interfering particles of rabies virus. Journal of Virology 21:626–635
    [Google Scholar]
  17. Wunner W. H., Clark H F. 1978; Study of virulent and avirulent rabies viruses and their defective RNA-containing particles. In Negative Strand Viruses and the Host Cell pp 599–606 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  18. Yoshino K., Taniguchi S., Arai K. 1966; Autointerference of rabies virus in chick embryo fibroblasts. Proceedings of the Society for Experimental Biology and Medicine 123387–392
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-51-1-69
Loading
/content/journal/jgv/10.1099/0022-1317-51-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error