1887

Abstract

SUMMARY

The nature of tubular structures (TS) specifically found in cells infected with herpes simplex virus type 2 (HSV-2) was investigated by studying the appearance of TS in mixed infections with herpes simplex virus type 1 (HSV-1) and HSV-2 in Vero cells. Mixed infections with HSV-1 and HSV-2 resulted in a notable reduction in TS appearance. Accumulation of TS in cells infected with u.v.-irradiated HSV-2 was decreased by superinfection with HSV-1. The majority of progeny viruses obtained from mixed infection with HSV-1 and HSV-2 was neutralized by type-specific anti-HSV-2 serum, but not by type-specific anti-HSV-1 serum. Analysis of the genotype of the progeny revealed that the yield from mixed infection contained both HSV-1 and HSV-2 genotypes. These observations indicate that the majority of HSV-1 progeny of mixed infection is phenotypically mixed and make it possible to propose that TS are materials related to the type-specific antigen for HSV-2 which can be utilized by superinfection with HSV-1, producing phenotypically HSV-2 particles.

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1980-11-01
2024-12-12
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References

  1. Aurelian L., Strandberg J. D., Melendez L. V., Johnson L. A. 1971; Herpesvirus type 2 isolated from cervical tumor cells grown in tissue culture. Science 174:704–707
    [Google Scholar]
  2. Couch E. F., Nahmias A. J. 1969; Filamentous structures of type 2 herpesvirus hominis infection of the chorioallantoic membrane. Journal of Virology 3:228–232
    [Google Scholar]
  3. Dunker R., Brown D. T. 1979; Tubular subviral structure produced in adenovirus-infected KB cells. Journal of Virology 31:568–574
    [Google Scholar]
  4. Glorioso J. C., Wilson L. A., Fenger T. W., Smith J. W. 1978; Complement-mediated cytolysis of HSV-1 and HSV-2 infected cells: plasma membrane antigens reactive with type-specific and crossreactive antibody. Journal of General Virology 40:443–454
    [Google Scholar]
  5. Iwasaka T., Miyazono J., Mori R. 1978; Unusual membranous structures found in cells infected with herpes simplex virus type 2 maintained in media containing 2-deoxy-d-glucose. Microbiology and Immunology 22:275–279
    [Google Scholar]
  6. Iwasaka T., Oda H., Mori R., Miyazono J., Nagafuchi S. 1979; Production of tubular structures in Vero cells infected with herpes simplex virus type 2: effects of ultraviolet light irradiation and antimetabolites. Journal of General Virology 44:57–67
    [Google Scholar]
  7. Mori R., Minamishima Y., Tasaki T., Oda H., Takade A. 1973; Hexagonal arrangement of filaments in herpes simplex virus type 2-infected cells. Japanese Journal of Microbiology 17:230–232
    [Google Scholar]
  8. Murphy F. A., Harrison A. K., Whitefield S. G. 1976; Intranuclear formation of filaments in herpes virus hominis infection of mice. Archiv für die gesamte Virusforschung 21:463–468
    [Google Scholar]
  9. Oda H., Mori R. 1976; Electron microscope observation on tubular structures in cells infected with herpes simplex virus type 2. Archives of Virology 50:159–168
    [Google Scholar]
  10. Oda H., Mori R., Miyazono J., Iwasaka T. 1979; Effects of antimetabolites on the production of tubular structures in Vero cells infected with herpes simplex virus type 2. Archives of Virology 62:175–187
    [Google Scholar]
  11. Purifoy D. J. M., Powell K. L. 1977; Interference between strains of type 1 and type 2 herpes simplex virus. Virology 77:84–94
    [Google Scholar]
  12. Schwartz J., Roizman B. 1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in HEp-2 cells: electron microscopy. Journal of Virology 4:879–889
    [Google Scholar]
  13. Tasaki T., Mori R., Minamishima Y., Oda H. 1975; Rezidivierende neonatale herpetische Infektion: Isolierung des Herpes-simplex virus Typ 2. Zeitschrift für Hautkrankheiten 50:69–71
    [Google Scholar]
  14. Young S. K., Rowe N. H., Sanderlin K. C. 1977; Herpes simplex virus type 1 and 2 in clinical infections: differences observed by electron microscopy. Journal of Infectious Diseases 135:486–489
    [Google Scholar]
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