1887

Abstract

SUMMARY

An antiserum to immune T-type mouse interferon was prepared in rabbits by repeated injections of interferon, induced by phytohaemagglutinin (PHA) in mouse spleen cells and purified to 1 × 10 units/mg protein. The antiserum neutralized mouse interferons synthesized in response to several T mitogens and Brucella organisms, and also the type II interferon present in the serum of BCG-sensitized mice after an injection of the specific antigen, tuberculin. All these interferons are thus antigenically related; they are also all unstable at pH 2. In contrast, the antiserum did not neutralize interferons induced by West Nile virus (WNV) and Newcastle disease virus (NDV) , or by lipopolysaccharide and Brucella organisms ; all these interferons are stable at pH 2. It also failed to neutralize a non-glycosylated virus interferon prepared in the presence of tunicamycin.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-50-1-225
1980-09-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/50/1/JV0500010225.html?itemId=/content/journal/jgv/10.1099/0022-1317-50-1-225&mimeType=html&fmt=ahah

References

  1. Berg K., Ogburn C. A., Paucker K., Morgensen E., Cantell K. 1975; Affinity chromatography of human leucocyte and diploid cell interferons on sepharose bound antibodies. Journal of Immunology 114:640–644
    [Google Scholar]
  2. Böhlen P., Stein S., Dairman W., Udenfriend S. 1973; Fluorimetric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
    [Google Scholar]
  3. Bousquet C., Canat P., Ramuz M., Serre A. 1978; Coopération entre cellules adherentes et lymphocytes T murins pour la synthése in vitro d’interferon Brucella-induit. Annates d’Immunologie 129C:715–719
    [Google Scholar]
  4. Bousquet-Ucla C., Wietzerbin J., Falcoff E. 1980; Studies on Brucella interferon, chromatographic behaviour and purification. Archives of Virology 63:57–68
    [Google Scholar]
  5. De Maeyer-Guignard J., Tovey M. G., Gresser L., De Maeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on Poly (U) and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  6. Falcoff R., Fontaine-Brouti D., Falcoff E. 1968; Purification partielle et caracterisation de l’interferon provenant de cerveau de souris. Annates d’lmmunologie 115:279–287
    [Google Scholar]
  7. Falcoff E., Falcoff R., Lebleu B., Revel M. 1973; Correlation between the antiviral effect of interferon treatment and the inhibition in vitro mRNA translation in non-infected L cells. Journal of Virology 12:421–43
    [Google Scholar]
  8. Fujisawa J., Iwakura Y., Kawade Y. 1978; Non-glycosylated mouse L cell interferon produced by the action of tunicamycin. Journal of Biological Chemistry 253:8677–8679
    [Google Scholar]
  9. Havell E. A., Bermon B., Ogburn C. A., Berg K., Paucker K., Vilcek J. 1975; Two antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  10. Johnson H. M., Baron S. 1976; The nature of the suppressive effect of interferon and interferon inducers on the ‘in vitro’ immune response. Cellular Immunology 25:106–115
    [Google Scholar]
  11. Paucker K. 1977; Antigenic properties. Texas Reports on Biology and Medicine 35:23–28
    [Google Scholar]
  12. Sipe J., De Maeyer-Guignard J., Fauconnik B., De Maeyer E. 1973; Purification of mouse interferon by affinity chromatography on a solid phase immunoabsorbent. Proceedings of the National Academy of Sciences of the United States of America 70:1037–1040
    [Google Scholar]
  13. Sonnenfeld G., Mandel A. D., Merigan T. C. 1977; The immunosuppressive effect of type II mouse interferon preparations on antibody production. Cellular Immunology 34:193–206
    [Google Scholar]
  14. Valle M. J., Bobrove A. M., Strober S., Merigan T. C. 1975; Immune specific production of interferon by human T cells in combined macrophage-lymphocyte cultures in response to herpes simplex antigen. Journal of Immunology 114:435–441
    [Google Scholar]
  15. Wallen C. W., Dean J. H., Lucas D. O. 1973; Interferon and the cellular immune response: separation of interferon-producing cells from DNA synthetic cells. Cellular Immunology 6:110–122
    [Google Scholar]
  16. Wietzerbin J., Falcoff R., Catinot L., Falcoff E. 1977a; Affinity chromatographic analysis of murine interferons induced by viruses and by T and B cell stimulants. Annales d’lmmunologie 128C:699–708
    [Google Scholar]
  17. Wietzerbin J., Stefanos S., Falcoff R., Falcoff E. 1977b; Some properties of interferons induced by stimulants of T and B lymphocytes. Texas Reports on Biology and Medicine 35:205–211
    [Google Scholar]
  18. Wietzerbin J., Stefanos S., Falcoff R., Lucero M., Catinot L., Falcoff E. 1978a; Immune interferon induced by phytohemagglutinin in nude mouse spleen cells. Infection and Immunity 21:966–972
    [Google Scholar]
  19. Wietzerbin J., Stefanos S., Lucero M., Falcoff E., Thang D. C., Thang M. N. 1978b; Presence of a polynucleotide binding site on murine immune interferon (T-type). Biochemical and Biophysical Research Communications 85:480–489
    [Google Scholar]
  20. Wietzerbin J., Stefanos S., Lucero M., Falcoff E., O’malley J., Sulkowski E. 1979; Physicochemical characterization and partial purification of mouse immune interferon. Journal of General Virology 44:773–781
    [Google Scholar]
  21. Youngner J. S., Salvin S. B. 1973; Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. Journal of Immunology m:1914–1922
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-1-225
Loading
/content/journal/jgv/10.1099/0022-1317-50-1-225
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error