1887

Abstract

Established lines of insect cells have been obtained only recently and their use in arbovirus studies is only beginning (Suitor, 1966; Converse & Nagle, 1967; Rehacek, 1968; Singh & Paul, 1968 ; Banerjee & Singh, 1968; Yunker & Cory, 1968). Most arbovirus growth reported in insect cell lines does not cause concomitant cytopathic effect (CPE) and plaque formation has not been reported before.

The cell line employed in this study originated from tissues of the mosquito (Singh, 1967). The cells were cultured in a manner identical to that described by Singh (1967) and complete monolayers were obtained about 5 days after subculture. Because this line contains cell types of various sizes, a single cloning was made in order to isolate the smallest cell type. Small coverslips were placed in Petri dishes and dilutions of cell suspensions added. After incubation, coverslips containing single colonies were transferred to tubes in order to increase the cell population.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-4-545
1969-12-01
2024-10-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/4/JV0050040545.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-4-545&mimeType=html&fmt=ahah

References

  1. Bannerjee K., Singh K. R. P. 1968; Establishment of carrier cultures of Aedes albopictus cell line infected with arboviruses. Indian J. med Res 56:812
    [Google Scholar]
  2. Converse J. L., Nagle S. C. Jun. 1967; Multiplication of yellow fever virus in insect tissue cell cultures. J. Virol 1:1096
    [Google Scholar]
  3. Rehacek J. 1968; The growth of arboviruses in mosquito cells in vitro. Acta virol. Prague 12:241
    [Google Scholar]
  4. Rohitayodhin S., Hammon W. McD. 1962; Studies on Japanese B encephalitis virus vaccines from tissue culture. II. Development of an attenuated strain of virus. J. Immun 89:589
    [Google Scholar]
  5. Singh K. R. P. 1967; Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L). Curr. Sci 36:506
    [Google Scholar]
  6. Singh K. R. P., Paul S. D. 1968a; Multiplication of arboviruses in cell lines from Aedes albopictus and Aedes aegypti. Curr. Sci 37:65
    [Google Scholar]
  7. Singh K. R. P., Paul S. D. 1968b; Susceptibility of Aedes albopictus and Aedes aegypti cell lines to infection by arbo and other viruses. Indian J. med. Res 56:815
    [Google Scholar]
  8. Suitor E. C. Jun. 1966; Growth of Japanese encephalitis virus in Grace’s continuous line of moth cells. Virology 30:143
    [Google Scholar]
  9. Yunker C. E., Cory J. 1968; Infection of Grace’s Antheraea cells with arboviruses. Am. J. trop. Med. Hyg 17:889
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-5-4-545
Loading
/content/journal/jgv/10.1099/0022-1317-5-4-545
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error