1887

Abstract

SUMMARY

By pulse-labelling cells infected by adenovirus type 5 with [H]thymidine, extraction of the DNA and equilibrium density gradient centrifugation in caesium chloride, viral and cellular DNA synthesis could be readily differentiated. In the experimental system used, cellular DNA synthesis was inhibited early in infection and virus DNA synthesis began about 2 to 3 hr later. These events were related chronologically to the production of the virus antigens. When arginine was withdrawn from the cell culture medium, the production of virus DNA and the inhibition of cellular DNA synthesis was still apparent.

By labelling at later times when only virus DNA was being synthesized, the fate of the label could be followed during virus maturation. The results suggested that virus DNA was associated with heterogeneous membrane-containing structures before being assembled into virus particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-3-339
1969-10-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/3/JV0050030339.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-3-339&mimeType=html&fmt=ahah

References

  1. Ben-Porat T., Kaplan A. S. 1965; Mechanism of inhibition of cellular DNA synthesis by pseudorabies virus. Virology 25:22
    [Google Scholar]
  2. Borun T. W., Scharff M. D., Robbins E. 1967; Preparation of mammalian polyribosomes with the detergent Nonidet P 40. Biochim. biophys. Acta 149:302
    [Google Scholar]
  3. Dulbecco R., Hartwell L. H., Vogt M. 1965; Induction of cellular DNA synthesis by polyoma virus. Proc. natn. Acad. Sci. U.S.A. 53:403
    [Google Scholar]
  4. Flamm W. G., Bond H. E., Burr H. E. 1966; Density gradient centrifugation of DNA in a fixed angle rotor: a higher order of resolution. Biochim. biophys. Acta 129:310
    [Google Scholar]
  5. Ginsberg H. S., Dixon M. K. 1961; Nucleic acid synthesis in types 4 and 5 adenovirus-infected HeLa cells. J. exp. Med 113:283
    [Google Scholar]
  6. Ginsberg H. S., Bello L. J., Levine A. J. 1967 In The Molecular Biology of Viruses Edited by Colter J. S., Paranchych W. p. 647 London: Academic Press;
    [Google Scholar]
  7. Green M. 1959; Biochemical studies on adenovirus multiplication. I. Stimulation of phosphorus incorporation into deoxyribonucleic acid and ribonucleic acid. Virology 9:343
    [Google Scholar]
  8. Green M. 1962; Studies on the biosynthesis of viral DNA. Cold Spring Harb. Symp. quant. Biol 37:219
    [Google Scholar]
  9. Hatanaka M., Dulbecco R. 1966; Induction of DNA synthesis in SV40. Proc. natn. Acad. Sci. U.S.A. 56:736
    [Google Scholar]
  10. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immunofluorescent technique. Virology 34:470
    [Google Scholar]
  11. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. J. phys. Chem 65:1138
    [Google Scholar]
  12. JoKLiK W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. J. molec. Biol 10:452
    [Google Scholar]
  13. Jungwirth C., Launer J. 1968; Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis. J. Virol 2:401
    [Google Scholar]
  14. Laver W. G., Pereira H. G., Russell W. C., Valentine R. C. 1968; Isolation of an internal component from adenovirus type 5. J. molec. Biol 37:379
    [Google Scholar]
  15. Ledinko N. 1967; Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by adenovirus 2 or 12. Cancer Res 27:1459
    [Google Scholar]
  16. Maizel J. V., White D. O., Scharff M. D. 1968; The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology 36:126
    [Google Scholar]
  17. Polasa H., Green M. 1965; Biochemical studies on adenovirus multiplication. VIII. Analysis of protein synthesis. Virology 35:68
    [Google Scholar]
  18. Russell W. C., Becker Y. 1968; A maturation factor for adenovirus. Virology 35:18
    [Google Scholar]
  19. Russell W. C., Laver W. G., Sanderson P. J. 1968; Internal components of adenovirus. Nature, Lond 219:1127
    [Google Scholar]
  20. Russell W. C., Valentine R. C., Pereira H. G. 1967; The effect of heat on the anatomy of the adenovirus. J. gen. Virol 1:509
    [Google Scholar]
  21. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967; Adenovirus antigens-a study of their properties and sequential development in infection. J. gen. Virol 1:495
    [Google Scholar]
  22. Valentine R. C., Pereira H. G. 1965; Antigens and structure of the adenovirus. J. molec. Biol 13:13
    [Google Scholar]
  23. Wilcox W. C., Ginsberg H. S. 1963; Protein synthesis in type 5 adenovirus-infected cells. Effect of p-fluorophenylalanine on synthesis of protein, nucleic acids, and infectious virus. Virology 20:269
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-5-3-339
Loading
/content/journal/jgv/10.1099/0022-1317-5-3-339
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error