1887

Abstract

Summary

A kinetic analysis of formation of Semliki Forest virus-specific RNAs revealed that 22 RNA was synthesized first and accumulated within or on a structure not disrupted by homogenization, but disrupted by detergent treatment, presumably a lipid-containing membrane or vesicle. When sampled at the end of the latent period of virus growth the 22 RNA consisted of a mixture of two RNAs, double- and single-stranded. The amount of double-stranded 22 RNA remained constant thereafter with no complementary RNA synthesized after the latent period of virus growth.

Appearing during the latter portion of the latent period were 26 RNAs and 42 virus RNA. The rate at which 26 RNA accumulated remained constant throughout infection thereafter, whereas that of 42 virus RNA was maximal at the time of maximal virus accumulation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-2-251
1969-09-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/2/JV0050020251.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-2-251&mimeType=html&fmt=ahah

References

  1. Acheson N. H., Tamm I. 1967; Replication of Semliki Forest virus: an electron microscopic study. Virology 32:128
    [Google Scholar]
  2. Bablanian R., Eggers H. J., Tamm I. 1965; Studies on the mechanism of poliovirus-induced cell damage. I. The relation between poliovirus-induced metabolic and morphological alterations in cultured cells. Virology 26:100
    [Google Scholar]
  3. Baltimore D. 1966; Purification and properties of poliovirus double-stranded ribonucleic acid. J. molec. Biol 18:421
    [Google Scholar]
  4. Ben-Ishai Z., Goldblum N., Becker Y. 1968; The intra-cellular site and sequence of Sindbis virus replication. J. gen. Virol 2:365
    [Google Scholar]
  5. Borst P., Weissmann C. 1965; Replication of viral RNA. VIII. Studies on the enzymatic mechanism of replication of MS 2 RNA. Proc. natn. Acad. Sci. U.S.A. 54:982
    [Google Scholar]
  6. Burge B. W., Pfefferkorn E. R. 1967; Temperature-sensitive mutants of Sindbis virus: Biochemical correlates of complementation. J. Virol 1:956
    [Google Scholar]
  7. Canellakis E. S. 1962; Metabolism of nucleic acids. Ann. Rev. Biochem 31:273
    [Google Scholar]
  8. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am. J. trop. Med. Hyg 7:561
    [Google Scholar]
  9. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med. 99:167
    [Google Scholar]
  10. Eagle H. 1959; Amino acid-metabolism in mammalian cell cultures. Science N.Y. 130:432
    [Google Scholar]
  11. Franklin R. M. 1966; Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc. natn. Acad. Sci. U.S.A. 55:1504
    [Google Scholar]
  12. Friedman R. M. 1968; Replicative intermediate of an arbovirus. J. Virol. 2:547
    [Google Scholar]
  13. Friedman R. M., Berezesky I. K. 1967; Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication. J. Virol. 1:374
    [Google Scholar]
  14. Friedman R. M., Levy H. B., Carter W. B. 1966; Replication of Semliki Forest virus: three forms of viral RNA produced during infection. Proc. natn. Acad. Sci. U.S.A. 56:440
    [Google Scholar]
  15. Geiduschek E. P., Moohr J. W., Weiss S. B. 1962; The secondary structure of complementary RNA. Proc. natn. Acad. Sci. U.S.A. 48:1078
    [Google Scholar]
  16. Gierer A., Schramm G. 1956; Die Infektiosität der Nucleinsäure aus Tabakmosaik-virus. Z. Naturf 11b:138
    [Google Scholar]
  17. Gomatos P. J. 1967; RNA synthesis in reovirus-infected L 929 mouse fibroblasts. Proc. natn. Acad. Sci. U.S.A. 58:1798
    [Google Scholar]
  18. Gomatos P. J. 1968; Reovirus-specific, single-stranded RNA’s synthesized in vitro with enzyme purified from reovirus-infected cells. J. molec. Biol. 37:423
    [Google Scholar]
  19. Jeanteur P., Amaldi F., Attardi G. 1968; Partial sequence analysis of ribosomal RNA from HeLa cells. II. Evidence for sequences of non-ribosomal type in 45 and 32 S ribosomal RNA- precursors. J. molec. Biol 33:757
    [Google Scholar]
  20. Kelly R. B., Sinsheimer R. L. 1967; The replication of bacteriophage MS 2. VIII. Fractionation of the ‘replicative intermediate’ using a BNC column. J. molec. Biol 29:229
    [Google Scholar]
  21. Mandel B. 1964; The extraction of ribonucleic acid from poliovirus by treatment with sodium dodecyl sulfate. Virology 22:360
    [Google Scholar]
  22. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. J. biol. Chem 236:1372
    [Google Scholar]
  23. Penman S. 1966; RNA metabolism in the HeLa cell nucleus. J. molec. Biol 17:117
    [Google Scholar]
  24. Sedat J. W., Kelly R. B., Sinsheimer R. L. 1967; Fractionation of nucleic acid on benzoylated- naphthoylated DEAE cellulose. J. molec. Biol 26:537
    [Google Scholar]
  25. Skehel J. J., Burke D. C. 1968; A temperature-sensitive event in interferon production. J. gen. Virol 3:191
    [Google Scholar]
  26. Sonnabend J. A., Martin E. M., Mecs E. 1967; Viral specific RNA’s in infected cells. Nature, Lond 213:365
    [Google Scholar]
  27. Sreevalsan I. T., Lockhart R. Z. Jun. 1966; HeterogeneousRNA’ soccurring during thereplication of western equine encephalomyelitis virus. Proc. natn. Acad. Sci. U.S.A. 55:974
    [Google Scholar]
  28. Sreevalsan T., Lockhart R. Z. Jun., Dodson M. L. Jun., Hartman K. A. 1968; Replication of western equine encephalomyelitis virus. I. Some chemical and physical characteristics of viral ribonucleic acid. J. Virol 2:558
    [Google Scholar]
  29. Stoker M., Abel P. 1962; Conditions affecting transformation by polyoma virus. Cold Spring Harb. Symp. quant. Biol 27:375–386
    [Google Scholar]
  30. Weissmann C., Feix G. 1966; Replication of viral RNA. XI. Synthesis of viral ‘minus’ strands in vitro. Proc. natn. Acad. Sci. U.S.A. 55:1264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-2-251
Loading
/content/journal/jgv/10.1099/0022-1317-5-2-251
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error