A kinetic analysis of formation of Semliki Forest virus-specific RNAs revealed that 22 RNA was synthesized first and accumulated within or on a structure not disrupted by homogenization, but disrupted by detergent treatment, presumably a lipid-containing membrane or vesicle. When sampled at the end of the latent period of virus growth the 22 RNA consisted of a mixture of two RNAs, double- and single-stranded. The amount of double-stranded 22 RNA remained constant thereafter with no complementary RNA synthesized after the latent period of virus growth.

Appearing during the latter portion of the latent period were 26 RNAs and 42 virus RNA. The rate at which 26 RNA accumulated remained constant throughout infection thereafter, whereas that of 42 virus RNA was maximal at the time of maximal virus accumulation.


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