1887

Abstract

SUMMARY

The translation of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected and the major capsid protein (mol. wt. 150000) was shown to be present among the products by immune precipitation. Optimum conditions for the stimulation of amino acid incorporation were determined and found to be similar for mRNA from both infected and mock-infected cells.

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1980-08-01
2022-01-16
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References

  1. Allen G. P., Bryans J. T. 1976; Cell-free synthesis of equine herpesvirus type 3 nucleocapsid polypeptides. Virology 69:751–762
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Busby D. W. G., House W., Macdonald J. R. 1964 In Virological Technique p 120 London: Churchill;
    [Google Scholar]
  4. Carrasco L. 1977; The inhibition of cell functions after viral infection. FEBS Letters 76:11–15
    [Google Scholar]
  5. Chantler J. K., Stevely W. S. 1973; Virus-induced proteins in pseudorabies-infected cells. I. Acid-extractable proteins of the nucleus. Journal of Virology 11:815–822
    [Google Scholar]
  6. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilised on a membrane. Journal of Molecular Biology 12:829–842
    [Google Scholar]
  7. Jaquemont B., Roizman B. 1975; Ribonucleic acid synthesis in cells infected with herpes simplex virus. XI. Characterisation of viral high molecular weight nuclear RNA. Journal of General Virology 29:155–165
    [Google Scholar]
  8. Johnson F. B., Blacklow N. R., Haggan M. D. 1972; Immunological reactivity of antisera prepared against the sodium dodecyl sulphate treated structural polypeptides of adenovirus-associated virus. Journal of Virology 9:1017–1026
    [Google Scholar]
  9. Kaplan A. S., Vatter A. E. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–407
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–684
    [Google Scholar]
  11. Marcu K., Dudock B. 1974; Characterisation of a highly efficient protein synthesising system derived from commercial wheat germ. Nucleic Acids Research 1:1385–1397
    [Google Scholar]
  12. Mendecki J., Lee S. Y., Brawerman G. 1972; Characteristics of the polyadenylic segment associated with messenger ribonucleic acid in mouse sarcoma 180 ascites cells. Biochemistry 11:792–798
    [Google Scholar]
  13. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  14. Preston C. M. 1977a; The cell-free synthesis of herpesvirus induced polypeptides. Virology 78:349–353
    [Google Scholar]
  15. Preston C. M. 1977b; Cell-free synthesis of herpes simplex virus-coded pyrimidine deoxyribonucleoside kinase enzyme. Journal of Virology 23:455–460
    [Google Scholar]
  16. Rakusanova T., Ben-Porat T., Himeno M., Kaplan A. S. 1971; Early functions of the genome of herpesvirus. I. Characterisation of the RNA synthesised in cycloheximide-treated infected cells. Virology 46:877–889
    [Google Scholar]
  17. Stevely W. S. 1975; Virus-induced proteins in pseudorabies infected cells. II. Proteins of the virion and nucleocapsid. Journal of Virology 16:944–950
    [Google Scholar]
  18. Stevely W. S. 1977; Inverted repetition in the chromosome of pseudorabies virus. Journal of Virology 22:232–234
    [Google Scholar]
  19. Stevely W. S., Mcgrath B. M. 1978; The effect of hypertonic conditions on protein synthesis in cells infected with herpesvirus. FEBS Letters 87:308–310
    [Google Scholar]
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