1887

Abstract

SUMMARY

The functional characteristics of lymphoid cells were investigated during acute and latent infection of mice with herpes simplex virus (HSV). Cytotoxic T cells were found in the draining lymph node (DLN) 4 days p.i. and had reached maximum activity between 6 and 9 days. After the 12th day and during the period of latent infection (> 20 days) no cytotoxic cell activity was observed. Cytotoxic activity could only be detected when the lymphoid cells had been cultured for a period of 3 days. In general, the cell killing was specific for syngeneic infected target cells, although some killing of uninfected targets was observed. In contrast to the cytotoxic response, DLN cells responding to HSV in a proliferation assay were detected towards the end of the acute phase and at least up to 9 months thereafter. The significance of these observations for the pathogenesis of HSV is discussed.

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/content/journal/jgv/10.1099/0022-1317-49-2-309
1980-08-01
2021-10-27
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References

  1. Adler W. H., Rabinowitz S. G. 1973; Host defenses during primary Venezuelan equine encephalomyelitis virus infection in mice. II. In vitro methods for the measurement and qualitation of the immune response. Journal of Immunology 110:1354–1362
    [Google Scholar]
  2. Brummer E., Vris T. W., Sherwood Lawrence H. 1977; A microculture system for the measurement of antigen-induced murine lymphocyte proliferation: advantages of 5 % horse serum and 5 x io−5 mercapto-ethanol. Journal of Immunological Methods 17:319–327
    [Google Scholar]
  3. Brunner K. T., Mauel J., Cerottini J.-C., Chapuis B. 1968; Quantitative assay of the lytic action of immune lymphoid cells on 51Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology 14:181–196
    [Google Scholar]
  4. Doherty P. C., Bennink J. R. 1979; Vaccinia-specific cytotoxic T-cell responses in the context of H-2 antigens not encountered in thymus may reflect aberrant recognition of a virus-H-2 complex. Journal of Experimental Medicine 149:150–157
    [Google Scholar]
  5. Doherty P. C., Zinkernagel R. M. 1974; T-cell mediated immunopathology in viral infections. Transplantation Reviews 19:89–120
    [Google Scholar]
  6. Ennis F. A., Martin W. J., Verbonitz M. W., Butchko G. M. 1977; Specificity studies on cytotoxic thymus derived lymphocytes reactive with influenza virus infected cells: evidence for dual recognition of H-2 and viral hemagglutinin antigens. Proceedings of the National Academy of Sciences of the United States of America 74:3006–3010
    [Google Scholar]
  7. Field H. J., Hill T. J. 1974; The pathogenesis of pseudorabies in mice following peripheral inoculation. Journal of General Virology 23:145–157
    [Google Scholar]
  8. Field H. J., Bell S. E., Elion G. B., Nash A. A., Wildy P. 1979; Effect of acycloguanosine treatment on acute and latent herpes simplex infections in mice. Antimicrobial Agents and Chemotherapy 15:554–561
    [Google Scholar]
  9. Finberg R., Burakoff S. J., Cantor H., Benacerraf B. 1978; Biological significance of alloreactivity: T cells stimulated by Sendai virus-coated syngeneic cells specifically lyse allogeneic target cells. Proceedings of the National Academy of Sciences of the United States of America 75:5145–5149
    [Google Scholar]
  10. Griffin D. E., Johnson R. T. 1973; Cellular immune response to viral infection: in vitro studies of lymphocytes from mice infected with Sindbis virus. Cellular Immunology 9:426–434
    [Google Scholar]
  11. Hellman A., Fowler A. K., Steinman H. G., Buzzard P. M. 1972; Studies of the blastogenic response of murine lymphocytes. III. Specific viral transformation. Proceedings of the Society for Experimental Biology and Medicine 141:106–109
    [Google Scholar]
  12. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a mouse model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  13. Lodmell D. L., Niwa A., Hayashr K., Notkins A. L. 1973; Prevention of cell-to-cell spread of herpes simplex virus by leukocytes. Journal of Experimental Medicine 137:706–720
    [Google Scholar]
  14. Nash A. A. 1976; Separation of lymphocyte sub-populations using antibodies attached to staphylococcal protein A coated surfaces. Journal of Immunological Methods 12:149–161
    [Google Scholar]
  15. Nash A. A., Gell P. G. H. 1980; Cell-mediated immunity in herpes simplex virus-infected mice: suppression of delayed hypersensitivity by an antigen specific B lymphocyte. Journal of General Virology 48:359–364
    [Google Scholar]
  16. Nash A. A., Field H. J., Quartey Papafio R. 1980; Cell-mediated immunity in herpes simplex virus-infected mice: induction, characterization and antiviral effects of delayed type hypersensitivity. Journal of General Virology 48:351–357
    [Google Scholar]
  17. Oakes J. E. 1975; Role for cell mediated immunity in the resistance of mice to subcutaneous herpes simplex infection. Infection and Immunity 12:166–172
    [Google Scholar]
  18. Pfizenmaier K., Starzinski-Powitz A., Rollinghoff M., Falke D., Wagner H. 1977; T cell mediated cytotoxicity against herpes simplex virus infected target cells. Nature, London 265:630–632
    [Google Scholar]
  19. Rager-Zisman B., Allison A. C. 1976; Mechanism of immunologic resistance to herpes simplex virus I (HSV-1) infection. Journal of Immunology 116:35–40
    [Google Scholar]
  20. Rager-zisman B., Bloom B. R. 1974; Immunological destruction of herpes simplex virus I infected cells. Nature, London 251:542–543
    [Google Scholar]
  21. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  22. Sethi K. K., Brandis H. 1977; Specifically immune mouse T-cells can destroy H-2 compatible murine target cells infected with herpes simplex virus types 1 and 2. Zeitschrift für Immunitatsforschung 150:162–173
    [Google Scholar]
  23. Stutman O., Shen F. W., Boyse E. A. 1977; Ly phenotype of T cells cytotoxic for syngeneic mouse mammary tumors: evidence for T cell interactions. Proceedings of the National Academy of Sciences of the United States of America 74:5667–5671
    [Google Scholar]
  24. Thouless M. E. 1972; Serological properties of thymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 17:307–315
    [Google Scholar]
  25. Woodward J. G., Fernandez P. A., Daynes R. A. 1979; Cell mediated immune responses to syngeneic UV-induced tumors. III. Requirement for a Ia+ macrophage in the in vitro differentiation of cytotoxic T lymphocytes. Journal of Immunology 122:1196–1202
    [Google Scholar]
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