Growth of Bacteriophage φX-174 at Elevated Temperatures

Clifton E. Dowell

doi:10.1099/0022-1317-49-1-41

Growth of Bacteriophage φX-174 at Elevated Temperatures

Clifton E. Dowell
First Published: 01 July 1980 https://doi.org/10.1099/0022-1317-49-1-41

Abstract

Summary

The replication of bacteriophage φX-174 is impaired at temperatures above 40 °C. Mutants (ht) that replicate at high temperature were isolated and partially characterized. Wild-type φX fails to grow at high temperature because, unlike the mutants, it does not make appreciable amounts of single-stranded (ss)DNA. An unusual form of ssDNA, not found in complete virions, is described.

Received: 16/11/1979
Accepted: 23/01/1980
Published Online: 01/07/1980

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Journal of General Virology

Volume 49, Issue 1

Research Article

Free

Growth of Bacteriophage φX-174 at Elevated Temperatures

Abstract

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