1887

Abstract

SUMMARY

Conditions are described for the large scale production of mouse interferons from Ehrlich ascites tumour cells cultured as monolayers in roller bottles. With the procedure reported here, we have used 50 to 65 600 cm roller bottles to produce routinely 2 × 10 to 3 × 10 International units of crude mouse interferon/week with a specific activity of 1 × 10 to 1.5 × 10 units/mg protein.

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1980-07-01
2021-10-17
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References

  1. Berthold W., Tan C., Tan Y. H. 1978; Purification and in vitro labeling of interferon from a human fibroblastoid cell line. Journal of Biological Chemistry 253:5206–5212
    [Google Scholar]
  2. Cabrer B., Taira H., Broeze R. J., Kempe T. D., Williams K., Slattery E., Konigsberg W. H., Lengyel P. 1979; Structural characteristics of interferons from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 254:3681–3684
    [Google Scholar]
  3. Cantell K., Paucker K. 1963; Quantitative studies on viral interference in suspended L cells. IV. Production and assay of interferon. Virology 21:11–21
    [Google Scholar]
  4. Demaeyer-Guignard J., Tovey M. G., Gresser I., Demaeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly(U)- and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  5. Fujisawa J., Iwakura Y., Kawade Y. 1978; Nonglycosylated mouse L cell interferon produced by the action of tunicamycin. Journal of Biological Chemistry 253:8677–8679
    [Google Scholar]
  6. Henderson D. R., Joklik W. K. 1978; The mechanism of interferon induction by UV-irradiated reovirus. Virology 91:389–406
    [Google Scholar]
  7. Henle W., Henle G., Deinhardt F., Bergs V. V. 1959; Studies on persistent infections in tissue cultures. IV. Evidence for the production of an interferon in MCN cells by myxoviruses. Journal of Experimental Medicine no:525–541
    [Google Scholar]
  8. Isaacs A., Burke D. C. 1958; Mode of action of interferons. Nature, London 182:1073–1074
    [Google Scholar]
  9. Isaacs A., Lindenmann J. 1957; Virus interference. I. The interferon. Proceedings of the Royal Society B 147:258–267
    [Google Scholar]
  10. Iwakura Y., Yonehara S., Kawade Y. 1978a; Purification of mouse L cell interferon. Essentially pure preparations with associated cell growth inhibitory activity. Journal of Biological Chemistry 253:5074–5079
    [Google Scholar]
  11. Iwakura Y., Yonehara S., Kawade Y. 1978b; Presence of a common structure in the two molecular species of mouse L cell interferon. Biochemical and Biophysical Research Communications 84:557–563
    [Google Scholar]
  12. Kawakita M., Cabrer B., Taira H., Rebello M., Slattery E., Weideli H., Lengyel P. 1978; Purification of interferon from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 253:598–602
    [Google Scholar]
  13. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  14. Knight E. Jun 1976; Interferon: purification and initial characterization from human diploid cells. Proceedings of the National Academy of Sciences of the United States of America 73:520–523
    [Google Scholar]
  15. Oie H. K., Gazdar A. F., Buckler C. E., Baron S. 1972; High interferon producing line of transformed murine cells. Journal of General Virology 17:107–109
    [Google Scholar]
  16. Rubinstein M., Rubinstein S., Familletti P. C., Miller R. S., Waldman A. A., Pestka S. 1979; Human leukocyte interferon: production, purification to homogeneity, and initial characterization. Proceedings of the National Academy of Sciences of the United States of America 76:640–644
    [Google Scholar]
  17. Sehgal P. B., Tamm I., Vilcek J. 1975; Human interferon production: superinduction by 5, 6-dichloro-I-β-D-ribofuranosylbenzimidazole. Science 190:282–284
    [Google Scholar]
  18. Slattery E., Taira H., Broeze R., Lengyel P. 1980; Mouse interferons: production by Ehrlich ascites tumour cells infected with Newcastle disease virus and its enhancement by theophylline. Journal of General Virology 49: (in the press)
    [Google Scholar]
  19. Stewart W. E., Gosser L. B. II, Lockart R. Z. Jun 1971; Priming: a nonantiviral function of interferon. Journal of Virology 7:792–801
    [Google Scholar]
  20. Taira H., Broeze R. J., Jayaram B. M., Lengyel P., Hunkapiller M., Hood L. E. 1980; Mouse interferons: NH2-terminal amino acid sequences of various species. Science 207:528–530
    [Google Scholar]
  21. Tan Y. H., Armstrong J. A., Ke Y. M., Ho M. 1970; Regulation of cellular interferon production: enhancement by antimetabolites. Proceedings of the National Academy of Sciences of the United States of America 67:464–471
    [Google Scholar]
  22. Tan Y. H., Armstrong J. A., Ho M. 1971; Accentuation of interferon production by metabolic inhibitors and its dependence on protein synthesis. Virology 44:503–509
    [Google Scholar]
  23. Tovey M. G., Begon-Lours J., Gresser I. 1974; A method for the large scale production of potent interferon preparations. Proceedings of the Society for Experimental Biology and Medicine 146:809–815
    [Google Scholar]
  24. Vilcek J., Rossman T. G., Varacalli F. 1969; Differential effects of actinomycin D and puromycin on the release of interferon induced by double-stranded RNA. Nature, London 222:682–684
    [Google Scholar]
  25. Wiranowska-Stewart M., Chudizo T., Stewart W. E. II 1977; Repeated ‘superinduction’ of interferon in human diploid fibroblast culture. Journal of General Virology 37:221–223
    [Google Scholar]
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