The lesion of the fowl plague virus (FPV) mutants 18 and 236 has been located in RNA segment 2 ( gene, corresponding to P3). After double-infection with these mutants and 90 or 93, which also carry a lesion in segment 2, plaques were formed at the non-permissive temperature (40 °C). These plaques cannot be passaged at 40 °C and exhibit a morphology which differs from those formed by the wild-type virus. The yield of infectious particles after double-infection shows a non-linear correlation between the plaque number and dilution, indicating that at least two particles are needed for infection of a cell. All experimental evidence points to an intracistronic complementation within the P3 protein.


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