It is proposed that the genome of human cytomegalovirus (HCMV) consists of two unique sequences, L and S, bounded by two sets of redundant sequences (P. Sheldrick unpublished data). In this arrangement the terminal sequences (TR and TR) are repeated in an internal inverted form (IR and IR) and delimit L and S. After restriction endonuclease cleavage of the DNA, four 0.5 and four 0.25 fragments are found, indicating that HCMV DNA preparations consist of four equimolar populations differing only in the relative orientation of the L and S components. Cleavage of the CMV DNA with the restriction endonucleases II, dIII and I results in 32, 27 and 21 fragments, respectively. The arrangement of these fragments has been determined using molecular hybridization techniques, identification of terminal fragments and the identification of linkage groups by double-digestion. In this report the physical maps for the restriction endonucleases II, dIII and I are presented.


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