1887

Abstract

SUMMARY

Cleavage sites for the restriction endonucleases and were mapped on rabbit poxvirus and vaccinia virus DNA. These physical maps were used to analyse the structural variations between the two DNAs. Two specific heterologous regions, symmetrically arranged at each end of the genomes, have been identified. Region 1, representing the exterior part of the terminal repetition, appears to contain unrelated sequences in each DNA and accounts for the difference in length of the two genomes. Region 2, separated from region 1 by a conserved part of the terminal repetition, is located at the transition from repeated to unique DNA sequences. Its overall length of about 4 megadaltons is well conserved and it contains individual DNA-specific as well as conserved restriction sites. The major central part of the genomes (over 100 megadaltons) contains very few, widely dispersed restriction site variations.

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1980-04-01
2023-02-08
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References

  1. Archard L. C., MacKett M. 1979; Restriction endonuclease analysis of red cowpox virus and its white pock variant. Journal of General Virology 45:51–63
    [Google Scholar]
  2. Berns K. I., Silverman C. 1970; Natural occurrence of cross-linked vaccinia virus deoxyribonucleic acid. Journal of Virology 5:299–304
    [Google Scholar]
  3. Boone R. F., Parr R. P., Moss B. 1979; Intermolecular duplexes formed from polyadenylated vaccinia virus RNA. Journal of Virology 30:365–374
    [Google Scholar]
  4. Cabrera C. V., Esteban M., McCarron R., McAllister W. T., Holowczak J. A. 1978; Vaccinia virus transcription: hybridization of mRNA to restriction fragments of vaccinia DNA. Virology 86:102–114
    [Google Scholar]
  5. Clements J. B., Watson R. J., Wilkie N. M. 1977; Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome. Cell 12:275–285
    [Google Scholar]
  6. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  7. Frost E., Williams J. 1978; Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue. Virology 91:39–50
    [Google Scholar]
  8. Garon C. F., Barbosa E., Moss B. 1978; Visualization of an inverted terminal repetition in vaccinia virus DNA. Proceedings of the National Academy of Sciences of the United States of America 75:4863–4867
    [Google Scholar]
  9. Geshelin P., Berns K. I. 1974; Characterization and localization of the naturally occurring cross-links in vaccinia virus DNA. Journal of Molecular Biology 88:785–796
    [Google Scholar]
  10. Grodzicker T., Williams J., Sharp P., Sambrook J. 1974; Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symposia on Quantitative Biology 39:439–446
    [Google Scholar]
  11. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  12. Lai C. J., Nathans D. 1974; Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of DNA. Virology 60:466–475
    [Google Scholar]
  13. Lillehaug J. R., Kleppe K. 1975; Effect of salts and polyamines on T4 polynucleotide kinase. Biochemistry 14:1225–1229
    [Google Scholar]
  14. Loening U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophoresis. Biochemical Journal 113:131–138
    [Google Scholar]
  15. MacKett M., Archard L. C. 1979; Conservation and variation in orthopoxvirus genome structure. Journal of General Virology 45:683–701
    [Google Scholar]
  16. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequences of the rightward operator of phage lambda. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
    [Google Scholar]
  17. Maxam A., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences of the United States of America 74:560–564
    [Google Scholar]
  18. McCarron R. J., Cabrera C. V., Esteban M., McAllister W. T., Holowczak J. A. 1978; Structure of vaccinia DNA: analysis of the viral genome by restriction endonucleases. Virology 86:88–101
    [Google Scholar]
  19. McFadden G., Dales S. 1979; Biogenesis of poxviruses: mirror-image deletions in vaccinia virus DNA. Cell 18:101–108
    [Google Scholar]
  20. Müller H. K., Witter R., Schaffner W., Schumperli D., Menna A., Wyler R. 1978; Comparison of five poxvirus genomes by analysis with restriction endonucleases Hin dIII, Bam I and Eco RI. Journal of General Virology 38:135–147
    [Google Scholar]
  21. Petterson U., Tibbetts C., Philipson L. 1976; Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. Journal of Molecular Biology 101:479–501
    [Google Scholar]
  22. Roberts R. J. 1978; Restriction and modification enzymes and their recognition sequences. Gene 4:183–193
    [Google Scholar]
  23. Sambrook J., Sugden B., Keller W., Sharp P. A. 1973; Transcription of simian virus 40: III. Mapping of ‘early’ and ‘late’ species of RNA. Proceedings of the National Academy of Sciences of the United States of America 70:3711–3715
    [Google Scholar]
  24. Sarov I., Becker Y. 1967; Studies on vaccinia virus DNA. Virology 33:369–375
    [Google Scholar]
  25. Schümperli D., Menna A., Wittek R. 1978; Rabbit poxvirus DNA: restriction maps for Xho I and Kpn I. Institut fur Virologie der Universität Zürich, Zürich, Switzerland, Internal communication No. 1/78
    [Google Scholar]
  26. Schümperli D., Menna A., Schwendimann F., Wittek R. 1979a; Eco RI restriction map of rabbit poxvirus DNA. I. The left part of the genome. Institut fur Virologie der Universität Zürich, Zürich, Switzerland, Internal Communication No. 1/79
    [Google Scholar]
  27. Schümperli D., Menna A., Schwendimann F., Wittek R. 1979b; Eco RI restriction map of rabbit poxvirus DNA. II. The right part of the genome. Institut fur Virologie der Universität Zurich, Zurich, Switzerland, Internal communication No>. 2/79
    [Google Scholar]
  28. Shenk T. E., Rhodes C., Rigby P. W. J., Berg P. 1974; Mapping of mutational alterations in DNA with S1 nuclease: the location of deletions insertions and temperature-sensitive mutations in SV 40. Cold Spring Harbor Symposia on Quantitative Biology 39:61–67
    [Google Scholar]
  29. Smith H. O., Birnstiel M. L. 1976; A simple method for DNA restriction site mapping. Nucleic Acids Research 3:2387–2398
    [Google Scholar]
  30. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  31. Stow N. D., Subak-sharpe J. H., Wilkie N. M. 1978; Physical mapping of herpes simplex virus type 1 mutations by marker rescue. Journal of Virology 28:182–192
    [Google Scholar]
  32. Wittek R., Menna A., Schümperli D., Stoffel S., Muller H. K., Wyler R. 1977; Hind III and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA. Journal of Virology 23:669–678
    [Google Scholar]
  33. Wittek R., Menna A., Müller H. K., Schümperli D., Boseley P. G., Wyler R. 1978a; Inverted terminal repeats in rabbit poxvirus and vaccinia virus DNA. Journal of Virology 28:171–181
    [Google Scholar]
  34. Wittek R., Müller H. K., Menna A., Wyler R. 1978b; Length heterogeneity in the DNA of vaccinia virus is eliminated on cloning the virus. FEBS Letters 90:41–46
    [Google Scholar]
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