1887

Abstract

SUMMARY

In attempts to infect cucumber protoplasts with cucumber mosaic virus (CMV) RNA, the use of polyethylene glycol as an uptake inducer proved successful. This method involves resuspending protoplasts in high concentrations of polyethylene glycol in the presence of RNA, followed by rapid dilution. Levels of infection obtained with CMV-RNA were more reproducible than those using virus nucleoprotein. This method was also suitable for the inoculation of protoplasts with tobacco ringspot virus RNA.

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/content/journal/jgv/10.1099/0022-1317-47-1-199
1980-03-01
2024-12-14
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References

  1. Beier H., Bruening G. 1976; Factors influencing the infection of cowpea protoplasts with cowpea mosaic virus RNA. Virology 72:363–369
    [Google Scholar]
  2. Cassells A. C., Barlass M. 1978; The initiation of TMV infection in isolated protoplasts by polyethylene glycol. Virology 87:459–462
    [Google Scholar]
  3. Cassells A. C., Barnett A., Barlass M. 1978; Effect of polyacrylic acid treatment on the susceptibility of Nicotiana tabacum cv. Xanthi nc. to tobacco mosaic virus. Physiological Plant Pathology 13:13–21
    [Google Scholar]
  4. Davidson R. L., Gerald P. S. 1976; Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic cell Genetics 2:165–176
    [Google Scholar]
  5. Dawson J. R. O., Dickerson P. E., King J. M., Sakai F., Trim A. R. H., Watts J. W. 1978; Improved methods for infection of plant protoplasts with viral ribonucleic acid. Zeitschrift für Naturforschung 33C:548–551
    [Google Scholar]
  6. Glimelius K., Wallin A., Eriksson T. 1978; Concanavalin A improves the polyethylene glycol method for fusing plant protoplasts. Physiologia Plantarum 44:92–96
    [Google Scholar]
  7. Maule A. J., Boulton M. I., Wood K. R. 1980; An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathologische Zeitschrift (in the press)
    [Google Scholar]
  8. Mühlbach H-P., Camacho-Henriquez A., Sanger H. L. 1977; Infection of tomato protoplasts by ribonucleic acid of tobacco mosaic virus and by virus. Phytopathologische Zeitschrift 90:289–305
    [Google Scholar]
  9. Okuno T., Furusawa I. 1978; Factors influencing the infection of barley mesophyll protoplasts with brome mosaic virus RNA. Journal of General Virology 41:63–75
    [Google Scholar]
  10. Salazar L. F., Harrison B. D. 1978; Host range and properties of potato black ringspot virus. Annals of Applied Biology 90:375–386
    [Google Scholar]
  11. Sarkar S., Upadhya M. D., Melchers G. 1974; A highly efficient method of inoculation of tobacco mesophyll protoplasts with ribonucleic acid of tobacco mosaic virus. Molecular and General Genetics 135:1–9
    [Google Scholar]
  12. Scott H. A. 1963; Purification of cucumber mosaic virus. Virology 20:103–106
    [Google Scholar]
  13. Stace-Smith R., Hansen A. J. 1974; Occurrence of tobacco ringspot virus in sweet cherry. Canadian Journal of Botany 52:1647–1651
    [Google Scholar]
  14. Tomlinson J. A., Carter A., Dale W. T., Simpson C. J. 1970; Weed plants as a source of cucumber mosaic virus. Annals of Applied Biology 66:11–16
    [Google Scholar]
  15. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining the viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
  16. Wilcockson J., Hull R. 1974; The rapid isolation of plant virus RNAs using sodium perchlorate. Journal of General Virology 23:107–111
    [Google Scholar]
  17. Wolstenholme J., Burgoyne R. D., Stephen J. 1977; Studies on the MgS04-induced cytoplasmic uptake of proteins by cells in culture. Experimental Cell Research 104377–388
    [Google Scholar]
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