Further Investigation of a Temperature-sensitive Strain of Tobacco Mosaic Virus: Its Behaviour in Tomato Leaf Epidermis

A temperature-sensitive (ts) strain of tobacco mosaic virus (TMV), Ls1, was further investigated with regard to its behaviour at permissive (22 °C) and nonpermissive (32 °C) temperatures at the primary infection sites in tomato leaf epidermis. Tomato leaflets were inoculated with Ls1 or a wild-type strain, L, cultured at 22 or 32 °C and the epidermis isolated; virus spread was followed by staining with fluorescent antibody after enzymic digestion of the upper cell wall. Profiles of epidermis stained with fluorescent antibody indicated that Ls1 multiplies in primary-infected cells but that it cannot move to the neighbouring cells at the nonpermissive temperature.