1887

Abstract

SUMMARY

Rauscher leukaemia virus (RLV), extensively purified by rate and density gradient centrifugation procedures, exhibited aminopeptidase (AP) activity. The amount of virion activity was about 0.005 times the specific activity of purified hog kidney aminopeptidase M (EC 3.4.11.2). The activity was also found in purified Moloney and Gross leukaemia viruses, but not in comparable gradient fractions of uninfected JLSV-9 cells. Furthermore replacement of serum by bovine serum albumin in the growth medium of virus producing cells did not affect the AP activity. These results indicate that murine leukaemia viruses (MuLV) possess a tightly bound AP activity which is present as a minor component of the virion preparation and is probably not due to host cell membrane or serum contamination. Characterization of the pH optimum and substrate specificity show the MuLV aminopeptidase activity is similar to but different from both hog kidney, leucine aminopeptidase (EC 3.4.11.1) and aminopeptidase M (EC 3.4.11.2).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-46-2-363
1980-02-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/46/2/JV0460020363.html?itemId=/content/journal/jgv/10.1099/0022-1317-46-2-363&mimeType=html&fmt=ahah

References

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. 1974; A proposed nomenclature for the virion proteins of oncogenic RNA virus. Virology 60:595–601
    [Google Scholar]
  2. Baltimore D. 1974; Tumor viruses. Cold Spring Harbor Symposia on Quantitative Biology 39:1187–1200
    [Google Scholar]
  3. Banerjee R. K., Racker E. 1977; Solubilization, purification and characterization of nucleoside triphosphatase from avian myeloblastosis virus. Journal of Biological Chemistry 252:6700–6706
    [Google Scholar]
  4. Bhatti A. R., Weber J. 1978; Protease of adenovirus type 2. In vitro processing of core protein. Biochemical and Biophysical Research Communications 81:973–979
    [Google Scholar]
  5. Delange R. J., Smith E. L. 1971; Leucine aminopeptidase and other A-terminal exopeptidases. In The Enzymes vol 3 pp 81–118 Edited by Boyer P. D. New York, London: Academic Press;
    [Google Scholar]
  6. Jamjoom O. A., Naso R. B., Arlinghaus R. B. 1976; Selective decrease in the rate of cleavage of an intracellular precursor to Rauscher leukemia virus P30 by treatment of infected cells with actinomycin D. Journal of Virology 19:1054–1072
    [Google Scholar]
  7. Johnson R. W., Perry A., Robinson O. R. Jun, Shibley G. P. 1976; Method for reproducible large-volume production and purification of Rauscher murine leukemia virus. Applied Environmental Microbiology 31:182–188
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Louvard D., Semeriva M., Maroux S. 1976; The brush border intestinal aminopeptidase, a transmembrane protein as probed by macromolecular photolabelling. Journal of Molecular Biology 106:1023–1035
    [Google Scholar]
  10. Lu A. H., Soong M. M., Wong P. K. Y. 1979; Maturation of Moloney murine leukemia virus. Virology 93:269–274
    [Google Scholar]
  11. Oroszlan S., Gilden R. V. 1970; Immune virolysis: effect of antibody and complement on C-type RNA virus. Science 168:1478–1480
    [Google Scholar]
  12. Oroszlan S., Henderson L. E., Stephenson J. R., Copeland T. D., Long C. W., Ihle J. N., Gilden R. V. 1978; Amino- and carboxyl-terminat amino acid sequences of proteins coded by gag gene of murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America 75:1404–1408
    [Google Scholar]
  13. Smith E. L., Spackman D. H. 1955; Leucine aminopeptidase. V. Activation, specificity, and mechanism of action. Journal of Biological Chemistry 212:271–299
    [Google Scholar]
  14. Strand M., August J. T. 1976; Structural proteins of RNA tumor viruses: purification of envelope, core and internal components. Journal of Biological Chemistry 251:559–564
    [Google Scholar]
  15. Syrewicz J. J., Naso R. B., Wang C. S., Arlinghaus R. B. 1972; Purification of large amounts of murine RNA tumor viruses produced in roller bottle cultures. Applied Microbiology 24:488–494
    [Google Scholar]
  16. Wachsmuth E. D., Fritze I., Pfleiderer G. 1966; An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzymic properties. Biochemistry 5:169–174
    [Google Scholar]
  17. Yoshinaka Y., Luftig R. B. 1977a; Murine leukemia virus morphogenesis: cleavage of P70 in vitro can be accompanied by a shift from a concentrically-coiled internal strand (‘immature’) to a collapsed (‘mature’) form of the virus core. Proceedings of the National Academy of Sciences of the United States of America 74:3446–3450
    [Google Scholar]
  18. Yoshinaka Y., Luftig R. B. 1977b; Partial characterization of a P70 proteolytic factor that is present in purified virions of Rauscher leukemia virus (RLV). Biochemical and Biophysical Research Communications 76:54–63
    [Google Scholar]
  19. Yoshinaka Y., Luftig R. B. 1977c; Properties of a P70 proteolytic factor of murine leukemia viruses. Cell 12:709–719
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-46-2-363
Loading
/content/journal/jgv/10.1099/0022-1317-46-2-363
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error