1887

Abstract

SUMMARY

We have isolated a new filamentous phage from , a pathogenic bacterium of citrus canker. This phage, designated as Cf, measures 10075 ± 985 × 10 nm in length and forms an unusually clear but tiny plaque on the host within 12 h p.i. Infection by Cf neither killed nor drastically prevented host cells from propagation. However, the rate of host cell division was severely retarded. This phage exhibited an exceedingly narrow host range and was unstable in conventional tris buffer and synthetic medium routinely used for the preparation of other filamentous phages. Based on nucleotide composition analysis (which also revealed that Cf contains an unusual nucleotide), thermal denaturation characteristics and the hydroxyapatite column elution pattern, the genome of Cf was shown to be single-stranded (ss) DNA. During phage multiplication in host cells single-stranded virus DNA, replicative form I and replicative form II were detected. As shown by acrylamide gel electrophoresis, the size and conformation of the virus, RFI and RFII DNA species, were the same as their counterparts of another filamentous phage Xf.

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1980-02-01
2022-08-10
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References

  1. Dai H., Chiang K-S., Kuo T. T. 1976; A filamentous phage Cf from Xanthomonas citri. Festischrift in Memorial of President Chiang Kai-Shek pp 411–426 Academia Sinica; Taipei, Republic of China:
    [Google Scholar]
  2. Helling R. B., Goodman M., Boyer H. W. 1974; Analysis of endonuclease R. EcoRI fragments of DNAfrom Lamboid bacteriophages and other viruses by agarose-gel electrophoresis. Journal of Virology 14:1235–1244
    [Google Scholar]
  3. Hirt B. 1967; Selective extraction of Polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  4. Hoffmann-Berling H., Durwald H., Beulke I. 1963; Ein Fadiger DNA-phage (fd) und ein spharischer RNS-phage (fr) wirtsspezifisch fur mannliche von fd Stamme von, E. coli. Ill Biologisches Verhalten von fd und fr. Zeitschrift fur Naturforschung 18b:893–898
    [Google Scholar]
  5. Khym J. X. 1975; Analytical system for rapid separation of tissue nucleotides at low pressure on conventional anion exchangers. Clinical Chemistry 21:1245–1252
    [Google Scholar]
  6. Kieras F., Chiang K-S. 1971; Characterization of DNA components from some colorless algae. Experimental Cell Research 64:89–96
    [Google Scholar]
  7. Kuo T. T., Huang T-C., Chow T-Y. 1969; A filamentous bacteriophage from Xanthomonas oryzae. Virology 39:548–555
    [Google Scholar]
  8. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods in Enzymology 12B:184–195
    [Google Scholar]
  9. Marvin D. A., Hoffmann-Berling H. 1963; Physical and chemical properties of two new small bacteriophages. Nature, London 197:517–518
    [Google Scholar]
  10. Marvin D. A., Hohn B. 1969; Filamentous bacterial viruses. Bacteriological Reviews 33:172–209
    [Google Scholar]
  11. Minamishima Y., Takeya K., Ohnishi Y., Amako K. 1968; Physicochemical and biological properties of fibrous Pseudomonas bacteriophages. Journal of Virology 2:208–213
    [Google Scholar]
  12. Panter R. A., Symons R. H. 1966; Isolation and properties of a DNA-containing rodshaped bacteriophage. Australian Journal of Biological Sciences 19:565–573
    [Google Scholar]
  13. Quesnel L. B. 1969; Methods of microculture. Methods in Microbiology 1:366–425
    [Google Scholar]
  14. Saliver W. O., Tzagoloff H., Pratt D. 1964; Some physical-chemical and biological properties of the shaped coliphage m-13. Virology 24:359–371
    [Google Scholar]
  15. Sinsheimer R. L. 1959; Purification and properties of bacteriophage β 6×174. Journal of Molecular Biology 1:37–42
    [Google Scholar]
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