1887

Abstract

SUMMARY

The synthesis of virus RNA and proteins in cells infected with two populations of defective virions [Pr(1)53 and Pr(2)53] which vary in the overall composition of their DNA, but which share some structural and biological characteristics, have been examined. The experiments were done under two sets of conditions: (1) at high multiplicity of infection. In this case, practically all the cells in the cultures were co-infected with defective and infectious virions; (2) at low multiplicity of infection. In this case, 75% of the cells in the cultures were infected with defective virions only and 25% were co-infected with defective and infectious virions. The relative abundance of RNA classes complementary to different regions of the virus genomes that were synthesized under various conditions of infection were determined by the Southern (1975) technique; the synthesis of virus proteins was determined by polyacrylamide gel electrophoresis (PAGE). In cells co-infected with standard and defective virions, RNA complementary to the regions that are reiterated in the defective genomes is present in larger amounts than in cells infected with standard virions alone, indicating that the genomes of the defective virions are transcribed. Furthermore, the transcriptional controls that operate normally in the infected cells also operate in cells co-infected with standard and defective virions. The over-abundant accumulation of transcripts of some regions of the virus genome in cells co-infected with defective virions is not necessarily accompanied by an overproduction of some virus proteins. No difference in the PAGE profiles of the proteins synthesized was detected in cells co-infected with Pr(1)53 and standard virions. However, cells co-infected with standard and Pr(2)53 overproduced three polypeptides. Transcription of the virus genome is detectable in cells infected with Pr(2)53 alone but not in cells infected with Pr(1)53 alone. Virus protein synthesis is also detectable under these conditions in Pr(2)-, but not in Pr(1)-infected cells. Thus, despite the similarities in the biological characteristics of the two populations of defective virions described previously, similarities with respect to the expression of their genomes were not found.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-46-1-119
1980-01-01
2022-07-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/46/1/JV0460010119.html?itemId=/content/journal/jgv/10.1099/0022-1317-46-1-119&mimeType=html&fmt=ahah

References

  1. Ben-Porat T., Kaplan A. S. 1975; Abnormal replication of the DNA of defective pseudorabies virion. In Oncogenesis and Herpesviruses vol II pp 97–102 Edited by de-The G. Epstein M. A., zur Hausen H. Lyon: IARC Scientific publications; No 11
    [Google Scholar]
  2. Ben-Porat T., Kaplan A. S. 1976; A comparison of two populations of defective interfering pseudorabies virus particles. Virology 72:471–479
    [Google Scholar]
  3. Ben-Porat T., Demarchi J. M., Kaplan A. S. 1974; Characterization of defective interfering viral particles present in a population of pseudorabies virions. Virology 61:29–37
    [Google Scholar]
  4. Ben-Porat T., Kervina M., Kaplan A. S. 1975; Early functions of the genome of herpesviruses. V. Serological Analysis of ‘Immediate-Early’ Proteins. Virology 65:355–362
    [Google Scholar]
  5. Ben-Porat T., Stehn B., Kaplan A. S. 1976; Fate of parental herpesvirus DNA. Virology 71:412–422
    [Google Scholar]
  6. Bonner R. A., Laskey A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  7. Demarchi J. M., Ben-Porat T., Kaplan A. S. 1979; Expression of the genome of noninfectious particles in stocks of standard and defective interfering pseudorabies virus. Virology 97:457–463
    [Google Scholar]
  8. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  9. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
    [Google Scholar]
  10. Feldman L., Rixon F., Jean J. H., Ben-Porat T., Kaplan A. S. 1979; Transcription of the genome of pseudorabies virus (a herpesvirus) is stringently controlled. Virology 97:316–327
    [Google Scholar]
  11. Frenkel J., Jacob N. R., Honess R. W., Hayward G. S., Roizman B. 1975; Anatomy of herpes simplex virus DNA. III. Characterization of defective DNA molecules and biological properties of virus populations containing them. Journal of Virology 16:153–167
    [Google Scholar]
  12. Kaplan A. S. 1957; A study of the herpes simplex virus-rabbit kidney cell system by the plaque technique. Virology 4:435–457
    [Google Scholar]
  13. Kaplan A. S., Ben-Porat T., Rubenstein A. S. 1976; On the mechanism of herpesvirus DNA replication and the genesis of defective particles. In Cancer Biology Vol III Epidemiology, molecular events, oncogenicity and therapy pp 61–86 Edited by Borek C., King D. W. New York: Stratton Intercontinental Medical Book Corp;
    [Google Scholar]
  14. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  15. Murray B. K., Biswal N., Bookout J. B., Lanford R. E., Courtney R. J., Melnick J. L. 1975; Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175. Intervirology 5:173–184
    [Google Scholar]
  16. Rakusanova T., Ben-Porat T., Himeno M., Kaplan A. S. 1971; Early functions of the genome of herpesvirus. I. Characterization of the RNA synthesized in cycloheximide-treated, infected cells. Virology 46:877–890
    [Google Scholar]
  17. Rixon F. J., Ben-Porat T. 1979; Structural evolution of the DNA of pseudorabies defective viral particles. Virology 97:151–163
    [Google Scholar]
  18. Rubenstein A. S., Kaplan A. S. 1975; Electron microscopic studies of the DNA of defective and standard pseudorabies virions. Virology 66:385–392
    [Google Scholar]
  19. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  20. Warner J. P., Knopf P. M., Rich A. 1963; A multiple ribosomal structure in protein synthesis. Proceedings of the National Academy of Sciences of the United States of America 49:122–129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-46-1-119
Loading
/content/journal/jgv/10.1099/0022-1317-46-1-119
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error