1887

Abstract

Summary

This report deals with physical characterization of the generalized transducing phage 5006M. The morphology of the phage is presented, the buoyant density was determined (1.491 g/ml) and the G + C content of the phage DNA was found to be 44%. The phage genome has a length of 14.8 µm and mol. wt. of 30.7 × 10. Denaturation mapping revealed non-random circular permutation of the phage DNA. The genome exhibits 3.6% terminal redundancy as shown by homoduplex analysis. The existence of concatemeric precursors of phage 5006M DNA is inferred and the results are interpreted in terms of a sequential headful packaging mechanism.

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/content/journal/jgv/10.1099/0022-1317-45-2-389
1979-11-01
2019-11-20
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-2-389
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