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Highly infective nucleic acid preparations were made from Nicotiana clevelandii leaves infected with carrot mottle virus (CMotV). The infective material had the properties of a single-stranded RNA but was less than 1% of the total RNA in the preparations. It had an apparent mol. wt. of about 1.5 to 1.6 × 106 in agarose-polyacrylamide gels and a sedimentation coefficient of about 28 to 30S in linear-log sucrose gradients. The infective RNA did not contain any considerable poly-adenylate sequence, nor did it require an associated protein for infectivity.