1887

Abstract

Summary

The sedimentation constant of simian virus type 38 (SV-38) DNA was estimated to be 31.6S. The intrinsic viscosity of DNA was on average 86.5 dl/g and the length of the molecule determined by electron microscopy was 10.6 µm. The average mol. wt., as determined by sedimentation and viscometry, was 21.5 × 10, which agreed well with the value derived from the length of the molecule (21.4 × 10) and with the value of 21.2 × 10 determined by the relative electrophoretic mobility of the DNA fragments produced by restriction endonucleases R1, I and II.

The buoyant density of the DNA in caesium chloride and caesium sulphate was 1.7185 and 1.4295 g/ml respectively. The melting temperature of the DNA in 1 × SSC was 93.5 °C. The GC content calculated from ρ and Tm values was 59.3%.

II cleaves SV-38 DNA at three sites producing four fragments with mol. wt.: A, 9.3 × 10; B, 5.6 × 10; C, 3.3 × 10; and D, 2.9 × 10. After treatment with R1 and I, SV-38 DNA is cleaved into five and six fragments respectively, with mol. wt. for R1 fragments: A, 8.2 × 10; B, 6.5 × 10; C, 4.0 × 10; D, 1.27 × 10; and E, 1.07 × 10, and for I fragments: A, 6.5 × 10; B, 5.4 × 10; C, 4.2 × 10; D, 2.8 × 10; E, 2.5 × 10 and F, 0.25 × 10. The sequence of fragments within the SV-38 DNA molecules for II was deduced to be BDCA, and for R1 - BCEAD.

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/content/journal/jgv/10.1099/0022-1317-44-1-69
1979-07-01
2019-12-15
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-1-69
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