1887

Abstract

SUMMARY

Rotavirus infected monkey kidney cells (LLC-MK) have been labelled with S-methionine in the presence of actinomycin D. The cells have been lysed with SDS and the polypeptides separated by discontinuous polyacrylamide gel electrophoresis. Rotavirus polypeptides began to appear 4 to 5 h p.i.; incorporation was maximum at 8 h, but all the polypeptides were still being made 15 to 18 h p.i. Tissue culture adapted calf rotavirus particles were labelled with S-methionine and the polypeptides compared with cell associated rotavirus polypeptides. There were four inner coat, four outer coat and three non-structural polypeptides. Several of the outer coat polypeptides have altered mol. wt. on maturation. The polypeptides of rotavirus from seven species (human, pig, calf, lamb, mouse, foal and rabbit) have been compared and their mol. wt. calculated. The polypeptides fell into the same relative groupings for each virus, but there were variations in the mol. wt. of most comparable polypeptides. The polypeptides of tissue culture adapted and non-adapted calf rotavirus from the same original isolate varied only in one of the non-structural polypeptides.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-1-187
1979-07-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/1/JV0440010187.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-1-187&mimeType=html&fmt=ahah

References

  1. Bridger J. C., Woode G. N. 1976; Characterization of two particle types of calf rotavirus. Journal of General Virology 31:245–250
    [Google Scholar]
  2. Bryden A. S., Davies H. A., Thouless M. E., Flewett T. H. 1977; Diagnosis of rotavirus infection by cell culture. Journal of Medical Microbiology 10:121–125
    [Google Scholar]
  3. Cohen J. 1977; Ribonucleic acid polymerase activity associated with purified calf rotavirus. Journal of General Virology 36:395–402
    [Google Scholar]
  4. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  5. Elias M. M. 1977; Separation and infectivity of two particle types of human rotavirus. Journal of General Virology 37:191–194
    [Google Scholar]
  6. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., van Kirk D. H., Chanock R. M., Kapikian A. Z. 1976; Differentiation of human and calf reovirus-like agents associated with diarrhea using polyacrylamide gel electrophoresis of RNA. Virology 74:86–92
    [Google Scholar]
  7. Newman J. F. E., Brown F., Bridger J. C., Woode G. N. 1975; Characterization of a rotavirus. Nature, London 258:631–633
    [Google Scholar]
  8. Ornstein L. 1964; Disc electrophoresis. I. Background and theory. Annals of the New York Academy of Sciences 121:321–349
    [Google Scholar]
  9. Rodger S. M., Schnagl R. D., Holmes I. H. 1977; Further biochemical characterization, including the detection of surface glycoproteins, of human, calf and simian rotaviruses. Journal of Virology 24:91–98
    [Google Scholar]
  10. Schnagl R. D., Holmes I. H. 1976; Characteristics of the genomes of human infantile enteritis virus (rotavirus). Journal of Virology 19:267–270
    [Google Scholar]
  11. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 9:143–159
    [Google Scholar]
  12. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. 1977; Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Archives of Virology 53:287–294
    [Google Scholar]
  13. Thouless M. E., Bryden A. S., Flewett T. H. 1978; Serotypes of human rotavirus. Lancet 1:39
    [Google Scholar]
  14. Todd D., McNulty M. S. 1976; Characterization of pig rotavirus RNA. Journal of General Virology 33:147–150
    [Google Scholar]
  15. Todd D., McNulty M. S. 1977; Biochemical studies on a reovirus-like agent (rotavirus) from lambs. Journal of Virology 21:1215–1218
    [Google Scholar]
  16. Verly E., Cohen J. 1977; Demonstration of size variation of RNA segments between different isolates of calf rotavirus. Journal of General Virology 35:583–586
    [Google Scholar]
  17. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  18. Zweerink H. J., Joklik W. K. 1970; Studies on the intracellular synthesis of reovirus-specified proteins. Virology 41:501–518
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-1-187
Loading
/content/journal/jgv/10.1099/0022-1317-44-1-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error