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Volume 43, Issue 3

Mechanism of Host Restriction of Adenovirus-associated Virus Replication in African Green Monkey Kidney Cells Free

Abstract

SUMMARY

Human adenovirus (Ad) serotypes provide an early factor(s) that is necessary for adenovirus-associated virus (AAV) multiplication in human cell lines. However, little, if any, AAV production occurs in primary African green monkey kidney (AGMK) cells co-infected with AAV and a helper human Ad (non-permissive infection), unless cells are additionally infected with SV40 (permissive infection). To determine the basis of the host restriction of AAV replication in AGMK cells, AAV DNA, RNA and protein synthesis were analysed under various conditions of infection. Hybridization reactions revealed no detectable AAV-specific DNA or RNA in infections with AAV alone or in combination with SV40. In co-infections with AAV and Ad5 or Ad7, the synthesis of both AAV- and Ad-specific DNA and RNA occurred without a significant rise in titre of either virus. During non-permissive infection, however, AAV DNA synthesis was abnormal in that an expected accumulation of single-stranded progeny molecules was not observed. Finally, although intact 20S AAV transcripts were present in the cytoplasm of AGMK cells during non-permissive infection (in amounts ranging from 50 to 80% of that found during permissive infection), AAV-specific polypeptides were not demonstrable by polyacrylamide gel electrophoresis. Taken together, these experiments indicate that the host restriction of AAV replication in AGMK cells is exerted at the level of translation of the single AAV messenger RNA. In addition, it appears that one or more of the AAV polypeptides specified by this message is required for the production of single-stranded AAV progeny DNA.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1979
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1979-06-01
2024-04-26
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References

  1. Atchison R. W., Casto B. C., Hammon M. D. W. 1965; Adenovirus-associated defective virus particles. Science 149:745–755
     [Google Scholar]
  2. Baum S. G., Wiese W. H., Reich P. R. 1968; Studies on the mechanism of enhancement of adenovirus 7 infection in African green monkey cells by simian virus 40: formation of adenovirus-specific RNA. Virology 34:374–376
     [Google Scholar]
  3. Baum S. G., Horwitz M. S., Maizel J. V. Jun 1972; Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. Journal of Virology 10:211–219
     [Google Scholar]
  4. Blacklow N. R., Hoggan M. D., Rowe W. P. 1967; Immunofluorescent studies of the potentiation of an adenovirus-associated virus by adenovirus 7. Journal of Experimental Medicine 125:755–765
     [Google Scholar]
  5. Bloom M. E., Rose J. A. 1978; Transcription of adenovirus-associated virus RNA in isolated KB cell nuclei. Virology 84:118–126
     [Google Scholar]
  6. Buller R. M. L., Rose J. A. 1978a; Characterization of adenovirus-associated virus-induced polypeptides in KB cells. Journal of Virology 25:331–338
     [Google Scholar]
  7. Buller R. M. L., Rose J. A. 1978b; Characterization of adeno-associated virus polypeptides synthesized in vivo and in vitro. In Paroviruses pp. 281–288 Edited by Ward D., Tattersall P. Cold Spring Harbor Laboratory: Western Printing Service Ltd;
     [Google Scholar]
  8. Carter B. J., Khoury G., Rose J. A. 1972; Adenovirus-associated virus multiplication. IX. Extent of transcription of the viral genome in vivo. Journal of Virology 10:1118–1125
     [Google Scholar]
  9. Carter B. J., Rose J. A. 1974; Transcription in vivo of a defective parvovirus: sedimentation and electrophoretic analysis of RNA synthesized by adenovirus-associated virus and its helper adenovirus. Virology 61:182–199
     [Google Scholar]
  10. Ensigner M. J., Ginsberg H. S. 1972; Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus. Journal of Virology 10:328–339
     [Google Scholar]
  11. Friedman M. P., Lyons M. J., Ginsberg H. S. 1970; Biochemical consequences of type 2 adenovirus and simian virus 40 double infections of African green monkey kidney cells. Journal of Virology 5:586–597
     [Google Scholar]
  12. Hoggan M. D., Blacklow N. R., Rowe W. P. 1966; Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proceedings of the National Academy of Sciences of the United States of America 55:1467–1474
     [Google Scholar]
  13. Klessig D. F., Anderson C. W. 1975; Block to multiplication of adenovirus serotype 2 in monkey cells. Journal of Virology 16:1650–1668
     [Google Scholar]
  14. Malmgren R. A., Rabson A. S., Carney P. G., Paul F. G. 1966; Immunofluorescence of African green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. Journal of Bacteriology 91:262
     [Google Scholar]
  15. O’Conor G. T., Rabson A. S., Berezesky L. K., Paul F. J. 1963; Mixed infections with simian virus 40 and adenovirus 12. Journal of the National Cancer Institute 31:903–905
     [Google Scholar]
  16. Rabson A. S., O’Conor G. T., Berezesky I. K., Paul F. J. 1964; Enhancement of adenovirus growth in African green monkey kidney cell cultures by SV40. Proceedings of the Society for Experimental Biology and Medicine 116:187–190
     [Google Scholar]
  17. Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. 1966; Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proceedings of the National Academy of Sciences of the United States of America 55:336–341
     [Google Scholar]
  18. Rhode S. L. III 1976; Replication process of the parvovirus H-1. V. Isolation and characterization of temperature-sensitive H-1 mutants defective in progeny DNA synthesis. Journal of Virology 17:659–667
     [Google Scholar]
  19. Rose J. A., Koczot F. 1971; Adenovirus-associated virus multiplication. VI. Base composition of the deoxyribonucleic acid strand species and strand-specific in vivo transcription. Journal of Virology 8:771–777
     [Google Scholar]
  20. Rose J. A., Koczot F. 1972; Adenovirus-associated virus multiplication. VII. Helper requirement for viral deoxyribonucleic acid and ribonucleic acid synthesis. Journal of Virology 10:1–8
     [Google Scholar]
  21. Rowe W. P., Baum S. G. 1965; Studies of adenovirus-SV40 hybrid viruses. II. Defectiveness of the hybrid particles. Journal of Experimental Medicine 122:955–966
     [Google Scholar]
  22. Straus S. E., Ginsberg H. S., Rose J. A. 1976a; DNA-minus temperature-sensitive mutants of adenovirus type 5 help adenovirus-associated virus replication. Journal of Virology 17:140–148
     [Google Scholar]
  23. Straus S. E., Sebring E. D., Rose J. A. 1976b; Concatemers of alternating plus and minus strands are intermediates in adenovirus-associated virus DNA synthesis. Proceedings of the National Academy of Sciences of the United States of America 73:742–746
     [Google Scholar]
  24. Warnaar S. O., Cohen J. A. 1966; A quantitative assay for DNA-DNA hybrids using membrane filters. Biochemical and Biophysical Research Communications 24:554–560
     [Google Scholar]
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Mechanism of Host Restriction of Adenovirus-associated Virus Replication in African Green Monkey Kidney Cells
J. Gen. Virol. 43, 663 (1979); https://doi.org/10.1099/0022-1317-43-3-663
/content/journal/jgv/10.1099/0022-1317-43-3-663
/content/journal/jgv/10.1099/0022-1317-43-3-663
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