1887

Abstract

SUMMARY

When L cells were treated with interferon and subsequently with poly(rI). poly(rC), there was a pronounced toxic effect. Most of the cells lysed, but some survived and grew at the same rate as control cells to yield cells which were as sensitive to the effects of interferon and poly(rI). poly(rC) as the original population. The proportion of surviving cells did not vary with either the cell cycle or the cell density. The treated cells produced interferon and some of the interferon was produced by the resistant cells.

Cells which had been X-irradiated before treatment with interferon and poly(rI).poly(rC) behaved similarly so cell division was not necessary for the development of toxicity. The toxic effect also developed when cells were enucleated with the aid of cytochalas in B after treatment with interferon, but not if they were enucleated before treatment. It is concluded that the nucleus is essential for interferon to exert its effect on the cells, but not for the development of cytotoxicity after the addition of poly(rI).poly(rC).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-3-553
1979-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/3/JV0430030553.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-3-553&mimeType=html&fmt=ahah

References

  1. Atkins G. J., Johnston M. D., Westmacott L. M., Burke D. C. 1974; Induction of interferon in chick cells by temperature-sensitive mutants of Sindbis virus. Journal of General Virology 25:381–390
    [Google Scholar]
  2. Atherton K. T., Burke D. C. 1975; Interferon induction by viruses and polynucleotides: a differential effect of camptothecin. Journal of General Virology 29:297–304
    [Google Scholar]
  3. Burke D. C., Veomett G. 1977; Enucleation and reconstruction of interferon-producing cells. Proceedings of the National Academy of Sciences of the United States of America 74:3391–3395
    [Google Scholar]
  4. De Clercq E., De Somer P. 1975; Are cytotoxicity and interferon-inducing activity of poly(I).poly(C) invariably linked in interferon-treated L cells?. Journal of General Virology 27:35–44
    [Google Scholar]
  5. De Maeyer-Guignard J., Tovey M. G., Gresser I., De Maeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly (U) and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  6. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  7. Gusella J., Geller R., Clarke B., Weeks V., Houseman D. 1976; Commitment to erythroid differentiation by Friend erythroleukaemia cells: a stochastic analysis. Cell 9:221–230
    [Google Scholar]
  8. Halbach M., Koschel K., Jungwirth C. 1978; Interferon enhances the fragility of lysosomes in L-929 mouse fibroblasts. Journal of General Virology 39:387–390
    [Google Scholar]
  9. Heremans H., Stewart W. E. II, Billiau A., De Somere P. 1976; Toxicity of poly (rI).poly (rC) for interferon-treated cells: an ultrastructural evaluation. Journal of General Virology 30:131–135
    [Google Scholar]
  10. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  11. Lee S. H. S., Rozee K. R. 1970; Variation of interferon production during the cell cycle. Applied Microbiology 20:11–15
    [Google Scholar]
  12. Littlefield J. W. 1964; Three degrees of guanylic acid-inosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature, London 203:1142–1144
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. MacPherson I., Montagnier L. 1964; Agar suspension culture for the selection assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  15. Radke K. L., Colby C., Kates J. R., Krider H. M., Prescott D. M. 1974; Establishment and maintenance of the interferon-induced antiviral state: studies in enucleated cells. Journal of Virology 13:623–630
    [Google Scholar]
  16. Stewart W. E. II, De Clercq E. 1974; Relationship of cytotoxicity and interferon-inducing activity of polyriboinosinic acid. polyribocytidylic acid to the molecular weights of the homopolymers. Journal of General Virology 23:83–89
    [Google Scholar]
  17. Stewart W. E. II, Gosser L. B., Lockart R. Z. Jun 1971a; Priming: a non-antiviral function of interferon. Journal of Virology 7:792–801
    [Google Scholar]
  18. Stewart W. E. II, Gosser L. B., Lockart R. Z. Jun 1971b; Distinguishing characteristics of the interferon responses of primary and continuous mouse cell cultures. Journal of General Virology 13:35–50
    [Google Scholar]
  19. Stewart W. E. II, De Clercq E., Billiau A., Desmyter J., De Somer P. 1972; Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic. polyribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
    [Google Scholar]
  20. Stewart W. E. II, De Clercq E., De Somer P., Berg K., Ogburn C. A., Paucker K. 1973a; Antiviral and non-antiviral activity of highly purified interferon. Nature New Biology 246:141–143
    [Google Scholar]
  21. Stewart W. E. II, De Clercq E., De Somer P. 1973b; Specificity of interferon-induced enhancement of toxicity for double-stranded ribonucleic acids. Journal of General Virology 18:237–246
    [Google Scholar]
  22. Veomett G., Shay J., Hough P. V. C., Prescott D. M. 1976 In Methods in Cell Biology vol 13 pp. 1–6 Edited by Prescott D. M. New York/London: Academic Press Inc;
    [Google Scholar]
  23. Youngner J. S., Hallum J. V. 1969; Inhibition of induction of interferon synthesis in L-cell pretreated with interferon. Virology 37:473–475
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-3-553
Loading
/content/journal/jgv/10.1099/0022-1317-43-3-553
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error