1887

Abstract

SUMMARY

Serum-depleted human foetal skin fibroblasts were stimulated by addition of 10% foetal calf serum to proliferate synchronously for at least one cell cycle. This proliferation was suppressed by leukocyte or fibroblast interferon (IF), which prolonged the G1 phase and diminished the rate of DNA synthesis during the S phase in a dose-dependent manner. When used in identical concentration, as judged in terms of units of antiviral activity, fibroblast IF had more pronounced effects on cell cycle events than leukocyte IF. Interferon exerted its effect in early G1, before the cells were irreversibly committed to DNA synthesis.

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1979-03-01
2024-04-26
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References

  1. Adams A., Strander H., Cantell K. 1975; Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
    [Google Scholar]
  2. Dahl H., Degre M. 1976; Human interferon and cell growth inhibition, I. Inhibitory effect of human interferon on the growth rate of cultured human cells. Acta pathologica et microbiologica Scandinavica 84:285–292
    [Google Scholar]
  3. Fuse A., Kuwata T. 1976; Effects of interferon on the human cell line, RSa: inhibition of macromolecular synthesis. Journal of General Virology 33:17–24
    [Google Scholar]
  4. Gresser I., Brouty-Boyé D., Thomas M. T. 1970a; Interferon and cell division: I. Inhibition of the multiplication of mouse cell leukemia L1210 cells in vitro by interferon preparations. Proceedings of the National Academy of Sciences of the United States of America 66:1052–1058
    [Google Scholar]
  5. Gresser I., Brouty-Boyé D., Thomas M.-T., Macieira-Coelho A. 1970b; Interferon and cell division Influence of various experimental conditions on the inhibition of L1210 cell multiplication in vitro by interferon preparations. Journal of The National Cancer Institute 45:1145–1153
    [Google Scholar]
  6. Hartzman R. I., Bach F. H., Thurman G. B., Sell K. W. 1972; Precipitation of radioactivity labeled samples: a semiautomatic multiple-sample processor. Cellular Immunology 4:182–186
    [Google Scholar]
  7. Havell E. A., Vilcek J. 1972; Production of high-titered interferon in cultures of human diploid cells. Antimicrobiological Agents and Chemotherapy 2:476–484
    [Google Scholar]
  8. Killander D., Lindahl P., Lundin L., Leary P., Gresser I. 1976; Relationship between the enhanced expression of histocompatibility antigens on interferon treated L 1210 cells and their position in the cell cycle. European Journal of Immunology 6:56–69
    [Google Scholar]
  9. Lindahl P. 1974; Influence of interferon on normal cell functions. Thesis University of Stockholm;
    [Google Scholar]
  10. Lindgren A., Westermark B., Pontén J. 1975; Serum stimulation of stationary human glia and glioma cells in culture. Experimental Cell Research 95:311–319
    [Google Scholar]
  11. Macieira-Coelho A., Brouty-Boyé D., Thomas M.-T., Gresser I. 1971; Interferon and cell division. III Effect of interferon on the division cycle of L 1210 cells in vitro. Journal of Cell Biology 48:415–419
    [Google Scholar]
  12. O’Shaugnessy M. V., Lee S. H., Rozee K. R. 1972; Interferon inhibition of DNA synthesis and cell division. Canadian Journal of Microbiology 18:145–151
    [Google Scholar]
  13. Pardee A. B. 1974; A restriction point for control of normal animal cell proliferation. Proceedings of the National Academy of Sciences of the United States of America 71:1286–1290
    [Google Scholar]
  14. Paucker K., Cantell K., Henle W. 1962; Quantitative studies on viral interference in suspended L cells. III. Effect of interfering viruses and interferon on the growth rate of cells. Virology 17:324–334
    [Google Scholar]
  15. Sokawa Y., Watanabe Y., Watanabe Y., Kawade Y. 1977; Interferon suppresses the transition of quiescent 3T3 cells to a growing state. Nature, London 268:236–238
    [Google Scholar]
  16. Smith J. A., Martin L. 1973; Do cells cycle?. Proceedings of the National Academy of Sciences of the United States of America 70:1263–1267
    [Google Scholar]
  17. Temin H. J. 1971; Stimulation by serum of multiplication stationary chicken cells. Journal of Cellular Physiology 78:161–170
    [Google Scholar]
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