1887

Abstract

SUMMARY

A thermodynamic approach has been used to measure the amount of haemagglutinin and matrix protein expressed at the surface of P815 cells infected for periods between 4.5 and 11 h with either WSN (HoN1) or JAP (HN) strains of type A influenza virus. This involved measuring the interaction of different concentrations of labelled (Fab) preparations of specific antibody with normal and infected cells. Assuming that one molecule of (Fab) bound to one molecule of antigen, values for the number of molecules of antigen/infected cell ranged from 7.6 × 10 to 1.7 × 10 for haemagglutinin and 1.3 × 10 to 1.1 × 10 for matrix protein. The ratio of haemagglutinin/matrix protein was lower for WSN-infected cells (1.7) than for JAP-infected cells (10). The same reagents were reacted with three purified A type virions, WSN, JAP and Port Chalmers (H3N2). Each preparation bound anti-matrix protein (Fab) though the value for haemagglutinin/matrix protein was much higher (66) than for infected cells and suggested that a virion may have a small number (about 12) of matrix protein molecules exposed though it was not excluded that the matrix protein detected was exposed only on damaged virions. Pre-treatment of infected cells with unlabelled reagent (anti-haemagglutinin) reduced the subsequent binding of the same labelled reagent but not the binding of the labelled matrix protein reagent and vice versa, suggesting that the haemagglutinin and matrix protein were not very close to each other on the cell surface.

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1979-03-01
2024-04-27
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References

  1. Ada G. L., Yap K. L. 1977; Matrix protein expressed at the surface of cells infected with influenza viruses. Immunochemistry 14:643–51
    [Google Scholar]
  2. Aymard-henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. 1973; Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bullet in of the World Health Organisation 48:199–202
    [Google Scholar]
  3. Biddison W. E., Doherty P. C., Webster R. G. 1977; Antibody to influenza matrix protein detects a common antigen on the surface of cells infected with type A influenza viruses. Journal of Experimental Medicine 146:690–697
    [Google Scholar]
  4. Braciale T. J. 1977a; Immunologic recognition of influenza-virus infected cells. I. Generation of a virus strain-specific and a cross-reactive subpopulation of cytotoxic T cells in the response to Type A influenza viruses of different sybtypes. Cellular Immunology 33:423–436
    [Google Scholar]
  5. Braciale T. J. 1977b; Immunologic recognition of influenza virus-infected cells. II. Expression of influenza A matrix protein on the infected cell surface and its role in recognition by cross-reactive cytotoxicT cells. Journal of Experimental Medicine 146:673–89
    [Google Scholar]
  6. Braciale G. L., Ada T., Yap K. L. 1978; Functional and structural considerations in the recognition of virus-infected cells by cytotoxic T lymphocytes. Contemporary Topics in Molecular Immunology 7: (in the press)
    [Google Scholar]
  7. Choppin P. W., Compans R. W. 1975; The structure of influenza virus. In The Influenza Viruses and Influenza pp 15–50 Edited by Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  8. Effros R. B., Doherty P. C., Gerhard W., Bennink J. 1977; Generation of both cross-reactive and virus specific T-cell populations after immunization with serologically distinct influenza A viruses. Journal of Experimental Medicine 14s:557–568
    [Google Scholar]
  9. Gardner I., Bowern N. A., Blanden K. V. 1974; Cell mediated cytotoxicity against ectromelia virus-infected target cells. I. Specificity and kinetics. European Journal of Immunology 4:63–67
    [Google Scholar]
  10. Goding J. W. Conjugation of antibodies with fluorochromes: modifications to the standard methods. Journal of Immunological Methods 13:215–226
    [Google Scholar]
  11. Klotz L. M. 1953; Protein interactions. In The Proteins pp 727–806 Edited by Neurath H., Bailey K. New York: Academic Press;
    [Google Scholar]
  12. Laver W. G. 1969; Purification of influenza virus. In Fundamental Techniques in Virology pp 82–86 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  13. Laver W. G., Webster R. G. 1976; Preparation and immunogenicity of an influenza virus hemagglutinin and neuraminidase subunit vaccine. Virology 69:511–522
    [Google Scholar]
  14. Mcconahey P. J., Dixon F. J. 1966; A method of trace iodination of proteins for immunologic studies. International Archives of Allergy 29:185–189
    [Google Scholar]
  15. Morgan C., Rifkind R. A., Rose H. M. 1962; The use of ferritin-conjugated antibodies in electron microscopic studies of influenza and vaccinia viruses. Cold Spring Harbor Symposium on Quantitative Biology 27:57–61
    [Google Scholar]
  16. Yap K. L., Ada G. L. 1978a; The recovery of mice from influenza virus infection: adoptive transfer of immunity with immune T lymphocytes. Scandinavian Journal of Immunology 7:389–397
    [Google Scholar]
  17. Yap K. L., Ada G. L. 1978b; The recovery of mice from influenza A virus infection: adoptive transfer of immunity with influenza virus-specific cytotoxic T lymphocytes recognising a common virion antigen. Scandinavian Journal of Immunology 8:413–420
    [Google Scholar]
  18. Yap K. L., Ada G. L., Mckenzie I. F. C. 1978; Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus. Nature, London 273:238–9
    [Google Scholar]
  19. Zinkernagel R. M., Doherty P. C. 1977; Major transplantation antigens, viruses and the specificity of surveillance T cells. In Contemporary Topics in Immunobiology vol 7 pp 179–214 Edited by Stutman O. New York and London: Plenum Press;
    [Google Scholar]
  20. Zweerink H. J., Courtneidge S. A., Skehel J. J., Crumpton M. J., Askonas B. A. 1977; Cytotoxic T cells kill influenza virus infected cells but do not distinguish between serologically distinct type A viruses. Nature, London 267:354–356
    [Google Scholar]
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