1887

Abstract

SUMMARY

The time interval between administering the serum and the virus was found to influence the results of the mouse protection test for foot-and-mouth disease antibodies. In particular, for both IgG and IgM antibodies to strain A virus, the mouse protection index increased from zero to a maximum at about 6 h and remained high for at least five days.

Variations in the antiserum concentration, on a log scale, had a proportional effect on the mouse protection index, if between 1 and 3. The constant of proportionality was unity for IgM and 2 for IgG antibody. Comparison with neutralization tests revealed essentially parallel neutralization curves. The lower serum titre in the protection test, if computed for less than 10 LD/dose, was accounted for by the simple dilution of the inoculated serum into the volume of the mouse. Consequently, in the low titre range, the same virus-antibody reaction and its effect are operable in each of the two tests. Analysis of literature data in which both the protection test and the neutralization test results were available on the same sera showed consistency with the above conclusions for both cattle and swine sera.

The protection test had a highly atypical survival pattern occurring at antibody concentrations expected to neutralize more than 10 LD/dose. The resulting dampening effect on virus titre is postulated to be caused by the excess antibody of the passive immunity test interfering with the spread of infection. The effect is analogous to an anomaly caused by not removing the inoculum in quantal tissue culture assays and it prevents quantification of antibody levels in strong sera.

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1979-03-01
2022-10-02
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References

  1. Andrewes C. H., Elford W. J. 1933; Observations on anti-phage sera. I: ‘The percentage law’. British Journal of Experimental Pathology 14:367–376
    [Google Scholar]
  2. Cunha R. G. 1960; Demonstration of immune response to foot-and-mouth disease vaccine in protection test in young adult mice. Proceedings of the Society for Experimental Biology and Medicine 103:700–703
    [Google Scholar]
  3. Cunha R. G., Baptista J. A. Jun, Serrao U. M., Torturella I. 1957; El uso de los ratones lactantes en la evaluacion de los anticuerpos contra el virus de la fiebre aftosa y su significacion inmologica. Gaceta Veterinaria, Buenos Aires 19:243–267
    [Google Scholar]
  4. Cunha R. G., Honigman M. N. 1963; A comparison of serum tests in mice for the detection of foot-and-mouth disease antibody. American Journal of Veterinary Research 24:371–375
    [Google Scholar]
  5. Cunliffe H. R., Graves J. H. 1963; Formalin-treated foot-and-mouth disease virus: comparison of two adjuvants in cattle. Canadian Journal of Comparative Veterinary Science 27:193–196
    [Google Scholar]
  6. Dulbecco R., Vogt M., Strickland A. G. R. 1956; A study of the basic aspect of neutralization of two animal viruses, Western equine encephalitis virus and poliomyelitis virus. Virology 2:162–205
    [Google Scholar]
  7. Finney D. J. 1964 In Statistical Methods in Biological Assay 2nd edition chapter 21 New York: Hafner Publishing Company;
    [Google Scholar]
  8. Gomes I., Astudillo V. 1975; Foot-and-mouth disease: evaluation of mouse protection test results in relation to cattle immunity. Boletin del centro panamericano de fiebre aftosa17–18 9–16
    [Google Scholar]
  9. Gomes I., Fernandez A. A., Auge De Mello P. 1972; Foot-and-mouth disease circulating antibodies in convalescent cattle. Bulletin Office International des Epizooties 77:731–741
    [Google Scholar]
  10. Mckercher P. D., Bachrach H. L. 1976; A foot-and-mouth disease vaccine for swine. Canadian Journal of Comparative Medicine 40:67–74
    [Google Scholar]
  11. Mckercher P. D., Graves J. H., Cunliffe H. R., Callis J. J., Auge De Mello P., Gomed I., Fernandez A. A., Fernandes M. V. 1975; Foot-and-mouth disease vaccines. II Studies on the duration of immunity in cattle and pigs. Boletin del centro panamericano de fiebre aftosa19–20 24–30
    [Google Scholar]
  12. Pearson E. S., Hartley H. O. 1958 In Biometrika Tables 2nd edition, vol 1 p 204 London: Cambridge University Press;
    [Google Scholar]
  13. Trautman R. 1976; Unified mass-action theory for virus neutralization and radioimmunology. Scandinavian Journal of Immunology 5:609–622
    [Google Scholar]
  14. Trautman R., Harris W. H. 1977; Modeling and computer simulation approach to the mechanism of foot-and-mouth disease virus neutralization assays. Scandinavian Journal of Immunology 6:831–841
    [Google Scholar]
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