1887

Abstract

SUMMARY

Miller’s technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-3-443
1979-03-01
2022-07-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/3/JV0420030443.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-3-443&mimeType=html&fmt=ahah

References

  1. Angelier N., Lacroix J. C. 1975; Complexes de transcription d’origines nucléolaire et chromosomique d’ovocytes de Pleurodeles waltlii et P. poireti (Amphibiens, Urodéles). Chromosoma 51:323–335
    [Google Scholar]
  2. Bachenheimer S., Darnell J. E. 1975; Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA. Proceedings of the National Academy of Sciences of the United States of America 72:4445–4449
    [Google Scholar]
  3. Burnett J. P., Harrington J. A. 1968; Simian adenovirus SA 7 DNA: chemical, physical and biological studies. Proceedings of the National Academy of Sciences of the United States of America 60:1023–1029
    [Google Scholar]
  4. Carter T. H., Blanton R. A. 1978; Possible role of the 72000-dalton DNA binding protein in regulation of adenovirus type 5 early gene expression. Journal of Virology 25:664–674
    [Google Scholar]
  5. Foe V. E., Wilkinson L. E., Laird C. D. 1976; Comparative organization of active transcription units in Oncopeltus fasciatus. Cell 9:131–146
    [Google Scholar]
  6. Ginsberg H. S., Bello L. J., Levine A. J. 1967; Control of biosynthesis of host macromolecules in cells infected with adenovirus. In The Molecular Biology of Viruses pp 547–572 Edited by Colter S. J., Paranchych W. New York: Academic Press;
    [Google Scholar]
  7. Girard M., Bouche J.-P., Marty L., Revet B., Berthelot N. 1977; Circular adenovirus DNA-protein complexes from infected Hela cell nuclei. Virology 83:34–55
    [Google Scholar]
  8. Goldberg S., Nevins J., Darnell J. E. 1978; Evidence from UV transcription mapping that late adenovirus type 2 mRNA is derived from a large precursor molecule. Journal of Virology 25:806–810
    [Google Scholar]
  9. Green M., Parsons J. T., Pina M., Fujinaga K., Caffier H., Landgraf-Leurs I. 1970; Transcription of adenovirus genes in productively infected and in transformed cells. Cold Spring Harbor Symposium on Quantitative Biology 35:803–818
    [Google Scholar]
  10. Kedinger C., Brison O., Chambon P. 1978a; Adenovirus type 2 replication complexes. (Abstract) Workshop on the Molecular Biology of Adenoviruses Orenäs Castle; Landskrona, Sweden: June 11–16
    [Google Scholar]
  11. Kedinger C., Brison O., Perrin F., Wilhelm J. 1978b; Structural analysis of viral replicative intermediates isolated from adenovirus type 2-infected Hela cell nuclei. Journal of Virology 26:364–379
    [Google Scholar]
  12. Lechner R.L., Kelly T. J. 1977; The structure of replicating adenovirus 2 DNA molecules. Cell 12:1007–1020
    [Google Scholar]
  13. Mcguire P. M., Swart C., Hodge L. D. 1972; Adenovirus messenger RNA in mammalian cells: failure of polyribosome association in the absence of nuclear cleavage. Proceedings of the National Academy of Sciences of the United States of America 69:1578–1582
    [Google Scholar]
  14. Mcknight S. L., Miller O. L. 1977; Electron microscopic analysis of chromatin replication in the cellular blastoderm Drosophila melanogaster embryo. Cell 12:795–804
    [Google Scholar]
  15. Miller O. L., Barken A. H. 1972; Morphological studies of transcription. In Gene Transcription in Reproductive Tissue pp 155–177 Edited by Diczfalusy E. Stockholm: Karolinska Institutet;
    [Google Scholar]
  16. Miller O. L., Beatty B. R. 1969; Visualization of nucleolar genes. Science, New York 164:955–957
    [Google Scholar]
  17. Miller O. L., Hodge L. D. 1975; Visualization of a unique nuclear adenovirus DNA-protein complex. Journal of Cell Biology 67:284a
    [Google Scholar]
  18. Moyne G., Pichard E., Bernhard W. 1978; Localization of simian adenovirus 7 (SA 7) transcription and replication in lytic infection. An ultracytochemical and autoradiographical study. Journal of General Virology 40:77–92
    [Google Scholar]
  19. Nevins J. R., Darnell J. E. 1978; Groups of adenovirus type 2 mRNA’s derived from a large primary transcript: probable nuclear origin and possible common 3′ ends. Journal of Virology 25:811–823
    [Google Scholar]
  20. Oda T., Nakamura T., Watanabe S. 1977; Transcription complexes of chromatin showing a beads-like structure of heterogeneous ribonucleoprotein chains. Journal of Electron Microscopy 26:203–207
    [Google Scholar]
  21. Philipson L., Lindberg U. 1974; Reproduction of adenoviruses. In Comprehensive Virology Vol 3 pp 143–227 Edited by FraenkelConrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  22. Puvion-Dutilleul F., Bachellerie J.-P., Bernadac A., Zalta I.-P. 1977a; Complexes de transcription dans les fractions subnucleaires isolees de cellules de Mammiferes: etude ultrastructurale. Comptes rendus des seances de l’ Académie des Sciences, Série D 284:663–666
    [Google Scholar]
  23. Puvion-Dutilleul F., Bachellerie J.-P., Zalta J.-P., Bernhard W. 1977b; Morphology of ribosomal transcription units in isolated subnuclear fractions of mammalian cells. Biologie Cellulaire 30:183–194
    [Google Scholar]
  24. Puvion-Dutilleul F., Bernadac A., Puvion E., Bernhard W. 1977c; Visualization of two different types of nuclear transcriptional complexes in rat liver cells. Journal of Ultrastructure Research 58:108–117
    [Google Scholar]
  25. Puvion-Dutilleul F., Puvion E., Bernhard W. 1978; Visualization of nonribosomal transcriptional complexes after cortisol stimulation of isolated rat liver cells. Journal of Ultrastructure Research 63:118–131
    [Google Scholar]
  26. Russell W. C. 1971; The acid-soluble components of adenovirus and of adenovirus-infected cells. Journal of General Virology 11:65–69
    [Google Scholar]
  27. Stevenin J., Devilliers G., Jacob M. 1976; Size heterogeneity of the structural units of brain nuclear ribonucleoprotein particles. Molecular Biology Reports 2:385–391
    [Google Scholar]
  28. Sussenbach J. S., Van Der Vliet P. C., Ellens D. J., Jansz H. S. 1972; Linear intermediates in the replication of adenovirus DNA. Nature New Biology 239:47–49
    [Google Scholar]
  29. Tallman G., Akers J. E., Burlingham B. T., Reeck G. R. 1977; Histone synthesis is not coupled to the replication of adenovirus DNA. Biochemical and Biophysical Research Communications 79:815–822
    [Google Scholar]
  30. Van-Der Vliet P. C., Levine A. J. 1973; DNA binding proteins specific for cells infected by Adenovirus. Nature New Biology 146:170–174
    [Google Scholar]
  31. Wall R., Philipson L., Darnell J. E. 1972; Processing of adenovirus specific nuclear RNA during virus replication. Virology 50:27–34
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-3-443
Loading
/content/journal/jgv/10.1099/0022-1317-42-3-443
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error